Click image to see more details
Product Info Summary
| SKU: | A04468 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Cytochrome P450 2A13 CYP2A13 Antibody
SKU/Catalog Number
A04468
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Cytochrome P450 2A13 CYP2A13 Antibody (Catalog # A04468). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 2A13 CYP2A13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04468)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human Cytochrome P450 2A13.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04468 is reactive to CYP2A13 in Human
Reconstitution
Calculated molecular weight
56688 MW
Background of CYP2A13
Exhibits a coumarin 7-hydroxylase activity. Active in the metabolic activation of hexamethylphosphoramide, N,N-dimethylaniline, 2'-methoxyacetophenone, N-nitrosomethylphenylamine, and the tobacco-specific carcinogen, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone. Possesses phenacetin O-deethylation activity.
Fernandez-Salguero P., Am. J. Hum. Genet. 57:651-660(1995).
Su T., cer Res. 60:5074-5079(2000).
Cauffiez C., m. Biophys. Res. Commun. 317:662-669(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04468 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HeLa cells, 293 cells and A549 cells, using Cytochrome P450 2A13 antibody A04468.
Protein Target Info & Infographic
Gene/Protein Information For CYP2A13 (Source: Uniprot.org, NCBI)
Gene Name
CYP2A13
Full Name
Cytochrome P450 2A13
Weight
56688 MW
Superfamily
cytochrome P450 family
Alternative Names
Cytochrome P450 2A13;1.14.14.1;CYPIIA13;CYP2A13; CYP2A13 CPAD, CYP2A, CYPIIA13 cytochrome P450 family 2 subfamily A member 13 cytochrome P450 2A13|cytochrome P450, family 2, subfamily A, polypeptide 13|cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP2A13, check out the CYP2A13 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP2A13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome P450 2A13 CYP2A13 Antibody (A04468)
Hello CJ!
No publications found for A04468
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Cytochrome P450 2A13 CYP2A13 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Cytochrome P450 2A13 CYP2A13 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Anti-Cytochrome P450 2A13 CYP2A13 Antibody
Question
Is this A04468 anti-Cytochrome P450 2A13 antibody reactive to the isotypes of CYP2A13?
B. Banerjee
Verified customer
Asked: 2020-04-16
Answer
The immunogen of A04468 anti-Cytochrome P450 2A13 antibody is Synthesized peptide derived from internal of human Cytochrome P450 2A13. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-16
Question
We are currently using anti-Cytochrome P450 2A13 antibody A04468 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-05
Answer
The anti-Cytochrome P450 2A13 antibody (A04468) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-05
Question
I was wanting to use your anti-Cytochrome P450 2A13 antibody for WB for human nasal cavity epithelium on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human nasal cavity epithelium identification?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
As indicated on the product datasheet, A04468 anti-Cytochrome P450 2A13 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human nasal cavity epithelium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-16
Question
Is a blocking peptide available for product anti-Cytochrome P450 2A13 antibody (A04468)?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
We do provide the blocking peptide for product anti-Cytochrome P450 2A13 antibody (A04468). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-28
Question
Will anti-Cytochrome P450 2A13 antibody A04468 work for WB with nasal cavity epithelium?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
According to the expression profile of nasal cavity epithelium, CYP2A13 is highly expressed in nasal cavity epithelium. So, it is likely that anti-Cytochrome P450 2A13 antibody A04468 will work for WB with nasal cavity epithelium.
Boster Scientific Support
Answered: 2019-11-06
Question
I see that the anti-Cytochrome P450 2A13 antibody A04468 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-30
Question
Would anti-Cytochrome P450 2A13 antibody A04468 work on bovine WB with nasal cavity epithelium?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
Our lab technicians have not validated anti-Cytochrome P450 2A13 antibody A04468 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-Cytochrome P450 2A13 antibody A04468 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine nasal cavity epithelium in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-12
Question
See below the WB image, lot number and protocol we used for nasal cavity epithelium using anti-Cytochrome P450 2A13 antibody A04468. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-11
Question
Does A04468 anti-Cytochrome P450 2A13 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
As indicated on the product datasheet, A04468 anti-Cytochrome P450 2A13 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-17
Question
Is there a BSA free version of anti-Cytochrome P450 2A13 antibody A04468 available?
Verified Customer
Verified customer
Asked: 2017-11-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cytochrome P450 2A13 antibody A04468 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-28
Question
I am interested in to test anti-Cytochrome P450 2A13 antibody A04468 on human nasal cavity epithelium for research purposes, then I may be interested in using anti-Cytochrome P450 2A13 antibody A04468 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
The products we sell, including anti-Cytochrome P450 2A13 antibody A04468, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for nasal cavity epithelium using anti-Cytochrome P450 2A13 antibody A04468. Let me know if you need anything else.
S. Johnson
Verified customer
Asked: 2016-08-26
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-08-26
Question
Can you help my question with product A04468, anti-Cytochrome P450 2A13 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
C. Krishna
Verified customer
Asked: 2014-11-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04468 anti-Cytochrome P450 2A13 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-11-27
