Anti-MSL2 Antibody Picoband™

Product Name

Anti-MSL2 Antibody Picoband™

View all MSL2L1 Antibodies

SKU/Catalog Number

A02712-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MSL2 Antibody Picoband™ catalog # A02712-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-MSL2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02712-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human MSL2 recombinant protein (Position: M1-C577).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02712-1 is reactive to MSL2 in Human

Applications

A02712-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

63 kDa

Calculated molecular weight

91791 MW

Background of MSL2L1

MSL2 (MSL Complex Subunit 2) is a protein coding gene located on human chromosome 3q22.3. MSL2 is also known as MSL-2, MSL2L1 and RNF184. The human MSL2 gene encodes a 62541 Da protein containing 577 amino acids. The MSL2 protein is ubiquitously expressed in bone marrow, lymph node and other tissues. Among its related pathways are Chromatin organization and Chromatin Regulation / Acetylation. MSL2 is related to ligase activity. MSL2 is associated with some diseases, such as Atrial Septal Defect 3.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of MSL2 using anti-MSL2 antibody (A02712-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSL2 antigen affinity purified polyclonal antibody (Catalog # A02712-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSL2 at approximately 63 kDa. The expected band size for MSL2 is at 63 kDa.

Click image to see more details

Figure 2. IF analysis of MSL2 using anti-MSL2 antibody (A02712-1).
MSL2 was detected in an immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MSL2 Antibody (A02712-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Click image to see more details

Figure 3. Flow Cytometry analysis of K562 cells using anti-MSL2 antibody (A02712-1).
Overlay histogram showing K562 cells stained with A02712-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSL2 Antibody (A02712-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For MSL2 (Source: Uniprot.org, NCBI)

Alternative Names

FLJ10546; KIAA1585FLJ54913; male-specific lethal 2 homolog (Drosophila); male-specific lethal 2 homolog; male-specific lethal 2-like 1 (Drosophila); Male-specific lethal 2-like 1; Male-specific lethal-2 homolog 1; MSL-2; MSL2L1; MSL2-like 1; PHNPCC4; PMS2; RING finger protein 184msl-2; RNF184 MSL2 MSL-2L1, RNF184, MSL2 MSL complex subunit 2 E3 ubiquitin-protein ligase MSL2|E3 ubiquitin-protein transferase MSL2|MSL2-like 1|male-specific lethal 2 homolog|male-specific lethal 2-like 1|male-specific lethal-2 homolog 1|ring finger protein 184

For more info on MSL2, check out the MSL2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MSL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the MSL2 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis