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Product Info Summary
| SKU: | A05545-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MSK2/RSK-B/RPS6KA4 Antibody Picoband™
SKU/Catalog Number
A05545-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MSK2/RSK-B/RPS6KA4 Antibody Picoband™ catalog # A05545-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MSK2/RSK-B/RPS6KA4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05545-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MSK2/RSK-B/RPS6KA4 recombinant protein (Position: Q302-S772).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05545-2 is reactive to RPS6KA4 in Human, Mouse, Rat
Applications
A05545-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
86 kDa
Calculated molecular weight
48608 MW
Background of MSK2
Ribosomal protein S6 kinase alpha-4 is an enzyme that in humans is encoded by the RPS6KA4 gene. This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MSK2/RSK-B/RPS6KA4 antigen affinity purified polyclonal antibody (Catalog # A05545-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MSK2/RSK-B/RPS6KA4 at approximately 86 kDa. The expected band size for MSK2/RSK-B/RPS6KA4 is at 86 kDa.
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Figure 2. IHC analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2).
MSK2/RSK-B/RPS6KA4 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2).
MSK2/RSK-B/RPS6KA4 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2).
MSK2/RSK-B/RPS6KA4 was detected in a paraffin-embedded section of human metaplasia of squamous cells of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2).
MSK2/RSK-B/RPS6KA4 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2).
MSK2/RSK-B/RPS6KA4 was detected in a paraffin-embedded section of human rectal moderately differentiated adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2).
MSK2/RSK-B/RPS6KA4 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 8. IHC analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2).
MSK2/RSK-B/RPS6KA4 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 9. IF analysis of MSK2/RSK-B/RPS6KA4 using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2).
MSK2/RSK-B/RPS6KA4 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 10. Flow Cytometry analysis of K562 cells using anti-MSK2/RSK-B/RPS6KA4 antibody (A05545-2).
Overlay histogram showing K562 cells stained with A05545-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MSK2/RSK-B/RPS6KA4 Antibody (A05545-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RPS6KA4 (Source: Uniprot.org, NCBI)
Gene Name
RPS6KA4
Full Name
Ribosomal protein S6 kinase alpha-4
Weight
48608 MW
Superfamily
protein kinase superfamily
Alternative Names
MSK2; ribosomal protein S6 kinase, 90kDa, polypeptide 4; RPS6KA4; RSKB RPS6KA4 MSK2, RSK-B, S6K-alpha-4 ribosomal protein S6 kinase A4 ribosomal protein S6 kinase alpha-4|90 kDa ribosomal protein S6 kinase 4|mitogen- and stress-activated protein kinase 2|nuclear mitogen- and stress-activated protein kinase 2|ribosomal protein S6 kinase, 90kDa, polypeptide 4|ribosomal protein kinase B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS6KA4, check out the RPS6KA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS6KA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MSK2/RSK-B/RPS6KA4 Antibody Picoband™ (A05545-2)
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