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Product Info Summary
| SKU: | M00140 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-MSH2 Monoclonal Antibody
SKU/Catalog Number
M00140
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MSH2 Monoclonal Antibody catalog # M00140. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MSH2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00140)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACIH-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MSH2 MSH2 (MutS homologue 2) forms the hMutS- Alpha dimer with MSH6 and is an essential component of the mismatch repair process. hMutS- Alpha is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins and the Rad50-Mre11-NBS1 complex.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00140 is reactive to MSH2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
25 kDa
Calculated molecular weight
78458 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00140 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
FC 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
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Western blot analysis of MSH2 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MSH2 (Source: Uniprot.org, NCBI)
Gene Name
MSH2
Full Name
DNA mismatch repair protein Msh2
Weight
78458 MW
Superfamily
DNA mismatch repair MutS family
Alternative Names
Matrix metalloproteinase-9;MMP-9;3.4.24.35;92 kDa gelatinase;92 kDa type IV collagenase;Gelatinase B;GELB;67 kDa matrix metalloproteinase-9;82 kDa matrix metalloproteinase-9;MMP9;CLG4B; MSH2 COCA1, FCC1, HNPCC, HNPCC1, LCFS2, MMRCS2, hMSH2 mutS homolog 2 DNA mismatch repair protein Msh2|DNA mismatch repair protein Msh2 transcript|mutS homolog 2, colon cancer, nonpolyposis type 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MSH2, check out the MSH2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MSH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MSH2 Monoclonal Antibody (M00140)
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No publications found for M00140
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-MSH2 Monoclonal Antibody
Question
I was wanting to use your anti-MSH2 Monoclonal antibody for WB for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
It shows on the product datasheet, M00140 anti-MSH2 Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-31
Question
I have a question about product M00140, anti-MSH2 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00140 anti-MSH2 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-14
Question
you antibody to test anti-MSH2 Monoclonal antibody M00140 on human blood for research purposes, then I may be interested in using anti-MSH2 Monoclonal antibody M00140 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
The products we sell, including anti-MSH2 Monoclonal antibody M00140, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-05
Question
Here is the WB image, lot number and protocol we used for blood using anti-MSH2 Monoclonal antibody M00140. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-28
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-MSH2 Monoclonal antibody M00140. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-12
Question
Is a blocking peptide available for product anti-MSH2 Monoclonal antibody (M00140)?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
We do provide the blocking peptide for product anti-MSH2 Monoclonal antibody (M00140). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-30
Question
Is this M00140 anti-MSH2 Monoclonal antibody reactive to the isotypes of MSH2?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
The immunogen of M00140 anti-MSH2 Monoclonal antibody is A synthesized peptide derived from human MSH2 MSH2 (MutS homologue 2) forms the hMutS- Alpha dimer with MSH6 and is an essential component of the mismatch repair process. hMutS- Alpha is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins and the Rad50-Mre11-NBS1 complex. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-26
Question
Is there a BSA free version of anti-MSH2 Monoclonal antibody M00140 available?
T. Jones
Verified customer
Asked: 2019-08-21
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-MSH2 Monoclonal antibody M00140 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-21
Question
Does anti-MSH2 Monoclonal antibody M00140 work for WB with blood?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
According to the expression profile of blood, MSH2 is highly expressed in blood. So, it is likely that anti-MSH2 Monoclonal antibody M00140 will work for WB with blood.
Boster Scientific Support
Answered: 2019-05-21
Question
We are currently using anti-MSH2 Monoclonal antibody M00140 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
S. Yang
Verified customer
Asked: 2018-08-17
Answer
The anti-MSH2 Monoclonal antibody (M00140) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-17
Question
My team were well pleased with the WB result of your anti-MSH2 Monoclonal antibody. However we have been able to see positive staining in uterus nucleus. using this antibody. Is that expected? Could you tell me where is MSH2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
From literature, uterus does express MSH2. Generally MSH2 expresses in nucleus. Regarding which tissues have MSH2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7923193, 8769132
Embryonic kidney, Pubmed ID: 17525332
Erythroleukemia, Pubmed ID: 23186163
Muscle, Pubmed ID: 15489334
Testis, Pubmed ID: 17974005
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-02-13
Question
Would M00140 anti-MSH2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-07
Answer
You can see on the product datasheet, M00140 anti-MSH2 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-07
Question
We have seen staining in human muscle. Are there any suggestions? Is anti-MSH2 Monoclonal antibody supposed to stain muscle positively?
Verified Customer
Verified customer
Asked: 2017-07-27
Answer
Based on literature muscle does express MSH2. Based on Uniprot.org, MSH2 is expressed in secondary oocyte, uterus, testis, muscle, blood, embryonic kidney, erythroleukemia, among other tissues. Regarding which tissues have MSH2 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7923193, 8769132
Embryonic kidney, Pubmed ID: 17525332
Erythroleukemia, Pubmed ID: 23186163
Muscle, Pubmed ID: 15489334
Testis, Pubmed ID: 17974005
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-07-27
Question
I see that the anti-MSH2 Monoclonal antibody M00140 works with WB, what is the protocol used to produce the result images on the product page?
N. Mitchell
Verified customer
Asked: 2014-03-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-03-17
