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Product Info Summary
| SKU: | A14183 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-MRPS5 Antibody
SKU/Catalog Number
A14183
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MRPS5 Antibody (Catalog # A14183). Tested in IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRPS5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14183)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MRPS5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14183 is reactive to MRPS5 in Human
Reconstitution
Calculated molecular weight
48006 MW
Background of MRPS5
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S5P family. Pseudogenes corresponding to this gene are found on chromosomes 4q, 5q, and 18q.
Suzuki T., J. Biol. Chem. 276:33181-33195(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14183 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human testis tissue using MRPS5 antibody A14183.
Protein Target Info & Infographic
Gene/Protein Information For MRPS5 (Source: Uniprot.org, NCBI)
Gene Name
MRPS5
Full Name
28S ribosomal protein S5, mitochondrial
Weight
48006 MW
Superfamily
universal ribosomal protein uS5 family
Alternative Names
28S ribosomal protein S5, mitochondrial;MRP-S5;S5mt;MRPS5; MRPS5 MRP-S5, S5mt mitochondrial ribosomal protein S5 28S ribosomal protein S5, mitochondrial|mitochondrial 28S ribosomal protein S5|mitochondrial small ribosomal subunit protein uS5m
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPS5, check out the MRPS5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPS5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRPS5 Antibody (A14183)
Hello CJ!
A14183 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Protective Effect of Dictyophora Polysaccharides on Sodium Arsenite-Induced Hepatotoxicity: A Proteomics Study
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-MRPS5 Antibody
Question
Does anti-MRPS5 antibody A14183 work on horse IHC with cerebellar hemisphere?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Our lab technicians have not tested anti-MRPS5 antibody A14183 on horse. You can run a BLAST between horse and the immunogen sequence of anti-MRPS5 antibody A14183 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cerebellar hemisphere in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-13
Question
Would A14183 anti-MRPS5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
You can see on the product datasheet, A14183 anti-MRPS5 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-06
Question
We are currently using anti-MRPS5 antibody A14183 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-03-01
Answer
The anti-MRPS5 antibody (A14183) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-03-01
Question
Is a blocking peptide available for product anti-MRPS5 antibody (A14183)?
B. Bhatt
Verified customer
Asked: 2014-08-04
Answer
We do provide the blocking peptide for product anti-MRPS5 antibody (A14183). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2014-08-04
