Anti-MRPL32 Antibody

Product Name

Anti-MRPL32 Antibody

View all MRPL32 Antibodies

SKU/Catalog Number

A14134

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MRPL32 Antibody (Catalog # A14134). Tested in WB, IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MRPL32 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14134)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human MRPL32.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A14134 is reactive to MRPL32 in Human

Reconstitution

Calculated molecular weight

21405 MW

Background of MRPL32

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp.

Suzuki T., J. Biol. Chem. 276:21724-21736(2001).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Kenmochi N., Genomics 77:65-70(2001).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A14134 is guaranteed for IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Immunofluorescence: 1:100~1:500

Validation Images & Assay Conditions

Gene/Protein Information For MRPL32 (Source: Uniprot.org, NCBI)

Alternative Names

39S ribosomal protein L32, mitochondrial;L32mt;MRP-L32;MRPL32;HSPC283; MRPL32 HSPC283, L32mt, MRP-L32, bMRP-59b mitochondrial ribosomal protein L32 39S ribosomal protein L32, mitochondrial|mitochondrial large ribosomal subunit protein bL32m

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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