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Product Info Summary
| SKU: | A10395 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MRPL12 Antibody
SKU/Catalog Number
A10395
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MRPL12 Antibody (Catalog # A10395). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRPL12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10395)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MRPL12.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10395 is reactive to MRPL12 in Human
Reconstitution
Calculated molecular weight
21348 MW
Background of MRPL12
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex.
Marty L., J. Biol. Chem. 271:11468-11476(1996).
Liu J., Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10395 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from COLO cells, using MRPL12 antibody A10395.
Protein Target Info & Infographic
Gene/Protein Information For MRPL12 (Source: Uniprot.org, NCBI)
Gene Name
MRPL12
Full Name
39S ribosomal protein L12, mitochondrial
Weight
21348 MW
Superfamily
bacterial ribosomal protein bL12 family
Alternative Names
39S ribosomal protein L12, mitochondrial;L12mt;MRP-L12;5c5-2;MRPL12;RPML12; MRPL12 5c5-2, L12mt, MRP-L31/34, MRPL7, MRPL7/L12, RPML12 mitochondrial ribosomal protein L12 39S ribosomal protein L12, mitochondrial|MRP-L12|mitochondrial large ribosomal subunit protein bL12m
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRPL12, check out the MRPL12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRPL12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRPL12 Antibody (A10395)
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