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Product Info Summary
| SKU: | A16446 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-MRGX4 MRGPRX4 Antibody
SKU/Catalog Number
A16446
Size
100μl
Form
Liquid
Description
Boster Bio Anti-MRGX4 MRGPRX4 Antibody catalog # A16446. Tested in ELISA, IF, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MRGX4 MRGPRX4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A16446)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
1B9C1
Isotype
IgG
Immunogen
Synthesized peptide derived from human MRGX4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A16446 is reactive to MRGPRX4 in Human
Applications
A16446 is guaranteed for ELISA, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
36461 MW
Background of MRGX4
BIVM (for basic, immunoglobulin-like variable motif-containing) refers to a recently identified gene product that maps to human chromosome 13q32-q33 and is predicted to encode a 503-amino acid protein. BIVM shows ubiquitous expression in normal human tissue and the presence of a 5' CpG island suggests it is a housekeeping gene. BIVM is likely essential for some aspect of basic cellular function. BIVM is highly charged and localizes to the cytoplasm and nucleus where it may bind to either DNA or RNA or associate with other cellular proteins. Significant sequence homology exists with many organisms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of HuvEc cells using MRGX4 Polyclonal antibody.
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Western blot analysis of lysates from HUVEC and COLO cells, using MRGX4 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HUVECcells using MRGX4 antibody.
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Immunofluorescence analysis of A549 cells, using MRGX4 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For MRGPRX4 (Source: Uniprot.org, NCBI)
Gene Name
MRGPRX4
Full Name
Mas-related G-protein coupled receptor member X4
Weight
36461 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
G protein-coupled receptor SNSR5; G protein-coupled receptor SNSR6; GPCR; MAS-related GPR, member X4; mas-related G-protein coupled receptor member X4; MGC129753; MGC129754; MRGPRX4; MRGX4; MRGX4G protein-coupled receptor MRGX4; Sensory neuron-specific G-protein coupled receptor 5/6; SNSR5; SNSR6 MRGPRX4 GPCR, MRGX4, SNSR6 MAS related GPR family member X4 mas-related G-protein coupled receptor member X4|G protein-coupled receptor MRGX4|G protein-coupled receptor SNSR5|G protein-coupled receptor SNSR6|MAS-related GPR, member X4|Mas-related G protein-coupled receptor X4|sensory neuron-specific G-protein coupled receptor 5/6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MRGPRX4, check out the MRGPRX4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MRGPRX4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MRGX4 MRGPRX4 Antibody (A16446)
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No publications found for A16446
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-MRGX4 MRGPRX4 Antibody
Question
Can you help my question with product A16446, anti-MRGX4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A16446 anti-MRGX4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-18
Question
Is this A16446 anti-MRGX4 antibody reactive to the isotypes of MRGPRX4?
J. Huang
Verified customer
Asked: 2015-12-23
Answer
The immunogen of A16446 anti-MRGX4 antibody is Synthesized peptide derived from the C-terminal region of human MRGX4. at AA rangle: 240-320. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-12-23
Question
See attached the WB image, lot number and protocol we used for middle temporal gyrus using anti-MRGX4 antibody A16446. Please let me know if you require anything else.
S. Parker
Verified customer
Asked: 2014-11-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-11-05
Question
I am interested in to test anti-MRGX4 antibody A16446 on human middle temporal gyrus for research purposes, then I may be interested in using anti-MRGX4 antibody A16446 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Walker
Verified customer
Asked: 2014-02-28
Answer
The products we sell, including anti-MRGX4 antibody A16446, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-02-28
