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Product Info Summary
| SKU: | M01345 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-(Mouse) Eed Antibody (Center)
SKU/Catalog Number
M01345
Size
80 µl
Description
Boster Bio Anti-(Mouse) Eed Antibody (Center) (Catalog # M01345). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-(Mouse) Eed Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01345)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This mouse Eed antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 269-303 amino acids from the Central region of mouse Eed.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01345 is reactive to Eed in Human, Mouse
Reconstitution
Calculated molecular weight
50198 Da
Background of EED
Polycomb group (PcG) protein. Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark (By similarity). The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity). Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8. Plays a role in X chromosome inactivation (XCI), in which one of the two X chromosomes in female mammals is transcriptionally silenced to equalize X-linked gene dosage with XY males. Required for stable maintenance of XCI in both embryonic and extraembryonic tissues. May prevent transcriptional activation of facultative heterochromatin during differentiation. Required for development of secondary trophoblast giant cells during placental development. May regulate hippocampal synaptic plasticity in the developing brain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01345 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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All lanes : Anti-Eed Antibody (Center) at 1:1000 dilution
Lane 1: A431 whole cell lysates
Lane 2: NIH/3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 50 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M01345 staining (Mouse) Eed in Mouse spleen tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For Eed (Source: Uniprot.org, NCBI)
Gene Name
Eed
Full Name
Polycomb protein EED
Weight
50198 Da
Superfamily
WD repeat ESC family
Alternative Names
Polycomb protein EED, Eed EED COGIS, HEED, WAIT1 embryonic ectoderm development polycomb protein EED|WD protein associating with integrin cytoplasmic tails 1|embryonic ectoderm development protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Eed, check out the Eed Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Eed: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-(Mouse) Eed Antibody (Center) (M01345)
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No publications found for M01345
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