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Product Info Summary
| SKU: | M01818 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD90 / Thy1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01818
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD90 / Thy1 Rabbit Monoclonal Antibody catalog # M01818. Tested in WB, IHC applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD90 / Thy1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01818)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CEB-20
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD90 / Thy1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01818 is reactive to THY1 in Human, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated molecular weight
17935 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01818 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
IHC: human tonsil tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of CD90 expression in Human fetal brain lysate.
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Immunohistochemical analysis of paraffin-embedded human tonsil, using CD90 / Thy1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For THY1 (Source: Uniprot.org, NCBI)
Gene Name
THY1
Full Name
Thy-1 membrane glycoprotein
Weight
17935 MW
Alternative Names
Thy-1 membrane glycoprotein;CDw90;Thy-1 antigen;CD90;THY1; THY1 CD90, CDw90 Thy-1 cell surface thy-1 membrane glycoprotein|Thy-1 T-cell |thy-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on THY1, check out the THY1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for THY1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD90 / Thy1 Rabbit Monoclonal Antibody (M01818)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CD90 / Thy1 Rabbit Monoclonal Antibody
Question
Is this M01818 anti-CD90 / Thy1 Rabbit Monoclonal antibody reactive to the isotypes of THY1?
S. Singh
Verified customer
Asked: 2020-03-27
Answer
The immunogen of M01818 anti-CD90 / Thy1 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD90 / Thy1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-27
Question
Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-13
Question
Can you help my question with product M01818, anti-CD90 / Thy1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01818 anti-CD90 / Thy1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-18
Question
Will anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 work for WB with leukemic t-cell?
L. Zhao
Verified customer
Asked: 2019-09-26
Answer
According to the expression profile of leukemic t-cell, THY1 is highly expressed in leukemic t-cell. So, it is likely that anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 will work for WB with leukemic t-cell.
Boster Scientific Support
Answered: 2019-09-26
Question
I see that the anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 works with WB, what is the protocol used to produce the result images on the product page?
O. Huang
Verified customer
Asked: 2019-09-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-17
Question
We bought anti-CD90 / Thy1 Rabbit Monoclonal antibody for IHC on leukemic t-cell last year. I am using rat, and I plan to use the antibody for WB next. I am interested in examining leukemic t-cell as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
I viewed the website and datasheets of our anti-CD90 / Thy1 Rabbit Monoclonal antibody and I see that M01818 has been tested on rat in both IHC and WB. Thus M01818 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-11
Question
Is a blocking peptide available for product anti-CD90 / Thy1 Rabbit Monoclonal antibody (M01818)?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
We do provide the blocking peptide for product anti-CD90 / Thy1 Rabbit Monoclonal antibody (M01818). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-29
Question
I was wanting to use your anti-CD90 / Thy1 Rabbit Monoclonal antibody for WB for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
You can see on the product datasheet, M01818 anti-CD90 / Thy1 Rabbit Monoclonal antibody has been tested for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-05-28
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-03-28
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-28
Question
We are currently using anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-25
Answer
The anti-CD90 / Thy1 Rabbit Monoclonal antibody (M01818) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-25
Question
My lab would like to test anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 on human leukemic t-cell for research purposes, then I may be interested in using anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-01-15
Answer
The products we sell, including anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-15
Question
Our team were content with the WB result of your anti-CD90 / Thy1 Rabbit Monoclonal antibody. However we have seen positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is THY1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-05-28
Answer
Based on literature, brain does express THY1. Generally THY1 expresses in cell membrane. Regarding which tissues have THY1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 10944468
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2018-05-28
Question
We have seen staining in rat leukemic t-cell. Do you have any suggestions? Is anti-CD90 / Thy1 Rabbit Monoclonal antibody supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2017-06-27
Answer
Based on literature leukemic t-cell does express THY1. Based on Uniprot.org, THY1 is expressed in anterior cingulate cortex, brain, amygdala, eye, liver, leukemic t-cell, among other tissues. Regarding which tissues have THY1 expression, here are a few articles citing expression in various tissues:
Amygdala, Pubmed ID: 17974005
Brain, Pubmed ID: 10944468
Eye, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2017-06-27
Question
Is there a BSA free version of anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 available?
J. Miller
Verified customer
Asked: 2015-04-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD90 / Thy1 Rabbit Monoclonal antibody M01818 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-04-13
Question
Would M01818 anti-CD90 / Thy1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Edwards
Verified customer
Asked: 2013-01-01
Answer
It shows on the product datasheet, M01818 anti-CD90 / Thy1 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-01-01
