Click image to see more details
Product Info Summary
| SKU: | FC01818-FITC |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD90 THY1 Monoclonal Antibody FITC Conjugated, Flow Validated
SKU/Catalog Number
FC01818-FITC
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD90 THY1 Monoclonal Antibody FITC Conjugated, Flow Validated (Catalog# FC01818-FITC). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD90 THY1 Monoclonal Antibody FITC Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01818-FITC)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: F15-42-1
Isotype
IgG1,k
Immunogen
Purified human brain Thy-1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC01818-FITC is reactive to THY1 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
18172 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC01818-FITC is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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Jurkat E6.1 cells stained with anti-human CD90 FITC (F15-42-1-5) (red histogram). Jurkat E6.1 cells stained with isotype control IgG1 MOPC-31C FITC (blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For THY1 (Source: Uniprot.org, NCBI)
Gene Name
THY1
Full Name
Thy-1 membrane glycoprotein
Weight
18172 MW
Alternative Names
Thy-1 membrane glycoprotein;Thy-1 antigen;CD90;Thy1;Thy-1; THY1 CD90, CDw90 Thy-1 cell surface thy-1 membrane glycoprotein|Thy-1 T-cell |thy-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on THY1, check out the THY1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for THY1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD90 THY1 Monoclonal Antibody FITC Conjugated, Flow Validated (FC01818-FITC)
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Anti-human CD90 THY1 Monoclonal Antibody FITC Conjugated, Flow Validated
Question
I see that the anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated FC01818-FITC works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
M. Johnson
Verified customer
Asked: 2020-02-06
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-06
Question
Does FC01818-FITC anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
It shows on the product datasheet, FC01818-FITC anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-12
Question
Here is the WB image, lot number and protocol we used for amygdala using anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated FC01818-FITC. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-22
Question
I was wanting to use your anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated for Flow Cytometry for human amygdala on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human amygdala identification?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
As indicated on the product datasheet, FC01818-FITC anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human amygdala in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-12
Question
I have a question about product FC01818-FITC, anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC01818-FITC anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-14
Question
I am interested in to test anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated FC01818-FITC on human amygdala for research purposes, then I may be interested in using anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated FC01818-FITC for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-04-12
Answer
The products we sell, including anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated FC01818-FITC, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-12
Question
Can FC00052-FITC, FC00555-FITC and FC01818-FITC be used on porcine?
Verified customer
Asked: 2019-03-28
Answer
Unfortunately, we do not have any data regarding potential porcine cross-reactivity for the Anti-Human CD44 Monoclonal Antibody FITC Conjugated, Flow Validated (FC00052-FITC), Anti-Human CD45 PTPRC Monoclonal Antibody FITC Conjugated, Flow Validated (FC00555-FITC) and Anti-Human CD90 THY1 Monoclonal Antibody FITC Conjugated, Flow Validated (FC01818-FITC).
Boster Scientific Support
Answered: 2019-04-01
Question
Is there a BSA free version of anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated FC01818-FITC available?
J. Jackson
Verified customer
Asked: 2018-12-26
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated FC01818-FITC are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-12-26
Question
Is a blocking peptide available for product anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated (FC01818-FITC)?
Verified Customer
Verified customer
Asked: 2018-12-04
Answer
We do provide the blocking peptide for product anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated (FC01818-FITC). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-04
Question
Does anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated FC01818-FITC work for Flow Cytometry with amygdala?
Verified Customer
Verified customer
Asked: 2018-12-04
Answer
According to the expression profile of amygdala, THY1 is highly expressed in amygdala. So, it is likely that anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated FC01818-FITC will work for Flow Cytometry with amygdala.
Boster Scientific Support
Answered: 2018-12-04
Question
Is this FC01818-FITC anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated reactive to the isotypes of THY1?
Verified Customer
Verified customer
Asked: 2017-11-29
Answer
The immunogen of FC01818-FITC anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated is Purified human brain Thy-1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for amygdala using anti-human CD90 Monoclonal antibody FITC Conjugated, Flow Validated FC01818-FITC. Let me know if you need anything else.
C. Krishna
Verified customer
Asked: 2014-03-21
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-21
