Product Info Summary
SKU: | A07628-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MOCS1 Antibody Picoband™
SKU/Catalog Number
A07628-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MOCS1 Antibody Picoband™ catalog # A07628-1. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MOCS1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07628-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MOCS1 recombinant protein (Position: E52-R84).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07628-1 is reactive to MOCS1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
58 kDa
Calculated molecular weight
157578 MW
Background of MOCS1
Molybdenum cofactor biosynthesis protein 1 is a protein that in humans and other animals, fungi, and cellular slime molds, is encoded by the MOCS1 gene. Molybdenum cofactor biosynthesis is a conserved pathway leading to the biological activation of molybdenum. The protein encoded by this gene is involved in this pathway. This gene was originally thought to produce a bicistronic mRNA with the potential to produce two proteins (MOCS1A and MOCS1B) from adjacent open reading frames. However, only the first open reading frame (MOCS1A) has been found to encode a protein from the putative bicistronic mRNA, whereas additional splice variants are likely to produce a fusion between the two open reading frames. This gene is defective in patients with molybdenum cofactor deficiency, type A. A related pseudogene has been identified on chromosome 16.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07628-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Immunohistochemistry, 2-5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
Validation Images & Assay Conditions
![a07628 1 mocs1 primary antibodies wb testing 1 a07628 1 mocs1 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a07628-1-mocs1-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of MOCS1 using anti-MOCS1 antibody (A07628-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human U20S whole cell lysates,
Lane 6: human 293T whole cell lysates,
Lane 7: human SH-SY5Y whole cell lysates,
Lane 8: human RT4 whole cell lysates,
Lane 9: rat liver tissue lysates,
Lane 10: rat PC-12 whole cell lysates,
Lane 11: mouse liver tissue lysates,
Lane 12: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOCS1 antigen affinity purified polyclonal antibody (Catalog # A07628-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MOCS1 at approximately 58 kDa. The expected band size for MOCS1 is at 70 kDa.
![a07628 1 mocs1 primary antibodies ihc testing 2 a07628 1 mocs1 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a07628-1-mocs1-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of MOCS1 using anti-MOCS1 antibody (A07628-1).
MOCS1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MOCS1 Antibody (A07628-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a07628 1 mocs1 primary antibodies ihc testing 3 a07628 1 mocs1 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a07628-1-mocs1-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of MOCS1 using anti-MOCS1 antibody (A07628-1).
MOCS1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MOCS1 Antibody (A07628-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a07628 1 mocs1 primary antibodies ihc testing 4 a07628 1 mocs1 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a07628-1-mocs1-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of MOCS1 using anti-MOCS1 antibody (A07628-1).
MOCS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MOCS1 Antibody (A07628-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a07628 1 mocs1 primary antibodies ihc testing 5 a07628 1 mocs1 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a07628-1-mocs1-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of MOCS1 using anti-MOCS1 antibody (A07628-1).
MOCS1 was detected in a paraffin-embedded section of human testicular germ cell tumors tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MOCS1 Antibody (A07628-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
![a07628 1 mocs1 primary antibodies if testing 6 a07628 1 mocs1 primary antibodies if testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a07628-1-mocs1-primary-antibodies-if-testing-6.jpg)
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Figure 6. IF analysis of MOCS1 using anti-MOCS1 antibody (A07628-1).
MOCS1 was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MOCS1 Antibody (A07628-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a07628 1 mocs1 primary antibodies fcm testing 7 a07628 1 mocs1 primary antibodies fcm testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a07628-1-mocs1-primary-antibodies-fcm-testing-7.png)
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Figure 7. Flow Cytometry analysis of K562 cells using anti-MOCS1 antibody (A07628-1).
Overlay histogram showing K562 cells stained with A07628-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MOCS1 Antibody (A07628-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MOCS1 (Source: Uniprot.org, NCBI)
Gene Name
MOCS1
Full Name
Molybdenum cofactor biosynthesis protein 1
Weight
157578 MW
Alternative Names
molybdenum cofactor synthesis 1 MOCS1 MIG11, MOCODA, MOCS1B, MOCS1 molybdenum cofactor synthesis 1 molybdenum cofactor biosynthesis protein 1|MOCS1A enzyme|cell migration-inducing gene 11 protein|molybdenum cofactor biosynthesis protein A|molybdenum cofactor synthesis-step 1 protein A-B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MOCS1, check out the MOCS1 Infographic
![MOCS1 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MOCS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MOCS1 Antibody Picoband™ (A07628-1)
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