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Product Info Summary
| SKU: | A03357-4 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-MNT Antibody Picoband®
View all MAX binding protein Antibodies
SKU/Catalog Number
A03357-4
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MNT Antibody Picoband® catalog # A03357-4. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MNT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03357-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MNT recombinant protein (Position: R222-A582).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03357-4 is reactive to MNT in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
62 kDa
Calculated molecular weight
62.3kDa
Background of MAX binding protein
MNT (Max-binding protein MNT) is a Max-binding protein that is encoded by the MNT gene The Myc/Max/Mad network comprises a group of transcription factors that co-interact to regulate gene-specific transcriptional activation or repression. This gene encodes a protein member of the Myc/Max/Mad network. This protein has a basic-Helix-Loop-Helix-zipper domain (bHLHzip) with which it binds the canonical DNA sequence CANNTG, known as the E box, following heterodimerization with Max proteins. This protein is likely a transcriptional repressor and an antagonist of Myc-dependent transcriptional activation and cell growth. This protein represses transcription by binding to DNA binding proteins at its N-terminal Sin3-interaction domain.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03357-4 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
ELISA, 0.1-0.5 µg/ml, Human
Positive Control
WB: human Hela whole cell, human A431 whole cell
ICC/IF: HELA cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MNT using anti-MNT antibody (A03357-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MNT antigen affinity purified polyclonal antibody (Catalog # A03357-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MNT at approximately 62 kDa. The expected band size for MNT is at 62 kDa.
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Figure 2. IF analysis of MNT using anti-MNT antibody (A03357-4) and anti-Beta Tubulin antibody (M01857-3).
MNT was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MNT Antibody (A03357-4) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For MNT (Source: Uniprot.org, NCBI)
Gene Name
MNT
Full Name
Max-binding protein MNT
Weight
62.3kDa
Alternative Names
Max-binding protein MNT; Class D basic helix-loop-helix protein 3; bHLHd3; Myc antagonist MNT; Protein ROX; MNT; BHLHD3; ROX MNT MAD6, MXD6, ROX, bHLHd3, lncRNA-HAL MAX network transcriptional repressor max-binding protein MNT|MAX binding protein|MNT, MAX dimerization protein|Max-interacting protein|class D basic helix-loop-helix protein 3|myc antagonist MNT
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MNT, check out the MNT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MNT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MNT Antibody Picoband® (A03357-4)
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