Click image to see more details
Product Info Summary
| SKU: | M00139 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MMP9 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00139
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MMP9 Rabbit Monoclonal Antibody catalog # M00139. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MMP9 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00139)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
BCD-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MMP9
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00139 is reactive to MMP9 in Human, Rat
Applications
M00139 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
25 kDa
Calculated molecular weight
78458 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of MMP9 expression in (1)Rat kidney tissue lysate;(2)Rat lung tissue lysate.
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Immunohistochemical analysis of paraffin-embedded human colon cancer, using MMP9 Antibody.
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Immunofluorescent analysis of U87-MG cells, using MMP9 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For MMP9 (Source: Uniprot.org, NCBI)
Gene Name
MMP9
Full Name
Matrix metalloproteinase-9
Weight
78458 MW
Superfamily
peptidase M10A family
Alternative Names
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase MMP9 CLG4B, GELB, MANDP2, MMP-9 matrix metallopeptidase 9 matrix metalloproteinase-9|macrophage gelatinase|matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|type V collagenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP9, check out the MMP9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MMP9 Rabbit Monoclonal Antibody (M00139)
Hello CJ!
M00139 has been cited in 31 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gao X,Qiao X,Xing X,Huang J,Qian J,Wang Y,Zhang Y,Zhang X,Li M,Cui J,Yang Y.Matrix Stiffness-Upregulated MicroRNA-17-5p Attenuates the Intervention Effects of Metformin on HCC Invasion and Metastasis by Targeting the PTEN/PI3K/Akt Pathway.Front Oncol.2020 Aug 19;10:1563.doi:10.3389/fonc.2020.01563.PMID:32974191;PMCID:PMC7466473.
Species: Human
M00139 usage in article: APP:IHC, SAMPLE:HCC CELL, DILUTION:1:50
Gao X,Qiao X,Xing X,Huang J,Qian J,Wang Y,Zhang Y,Zhang X,Li M,Cui J,Yang Y.Matrix Stiffness-Upregulated MicroRNA-17-5p Attenuates the Intervention Effects of Metformin on HCC Invasion and Metastasis by Targeting the PTEN/PI3K/Akt Pathway.Front Oncol.2020
Species: Human
M00139 usage in article: APP:IHC, SAMPLE:HCC CELL, DILUTION:1:50
Li CH,Liu M,Pan LH,Sun Y.ANP reduced Hedgehog signaling-mediated activation of matrix metalloproteinase-9 in gastric cancer cell line MGC-803.Gene.2020 Dec 15;762:145044.doi:10.1016/j.gene.2020.145044.Epub 2020 Aug 7.PMID:32777528.
Species: Human
M00139 usage in article: APP:WB, SAMPLE:MGC-803 CELL, DILUTION:1:400
ACS Appl.Bio Mater.2020,XXXX,XXX,XXX-XXX Publication Date:December 15, 2020 https://doi.org/10.1021/acsabm.0c00927
Species: Human,Mouse
M00139 usage in article: APP:IF, SAMPLE:4T1 CELL AND HUVECS, DILUTION:1:200
Liang Q,Lin Q,Li Y,Luo W,Huang X,Jiang Y,Qin C,Nong J,Chen X,Sooranna SR,Pinhu L.Effect of SIS3 on Extracellular Matrix Remodeling and Repair in a Lipopolysaccharide-Induced ARDS Rat Model.J Immunol Res.2020 Nov 25;2020:6644687.doi:10.1155/2020/6644687.PM
Species: Rat
M00139 usage in article: APP:IHC, SAMPLE:LUNG TISSUE, DILUTION:1:50
Ketamine inhibits lipopolysaccharide-induced astrocytes activation by suppressing TLR4/NF-%u0138B pathway
1-calcium phosphate-uracil, a synthesized pyrimidine derivative agent, has anti-proliferative, pro-apoptotic and anti-invasion effects on multiple tumor cell lines
Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-?/ERK1/2 pathway: An in vitro investigation
Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats
Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer
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Customer Reviews
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Customer Q&As
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18 Customer Q&As for Anti-MMP9 Rabbit Monoclonal Antibody
Question
I see that the anti-MMP9 Rabbit Monoclonal antibody M00139 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-21
Question
What secondary antibody would best work for Anti-MMP9 Rabbit Monoclonal Antibody for flow cytometry?
Verified customer
Asked: 2019-11-25
Answer
The recommended for M00139 for flow cytometry is BA1127 Goat Anti-Rabbit IgG (H+L) Secondary Antibody, DyLight®488 Conjugate.
Boster Scientific Support
Answered: 2019-11-27
Question
Is this M00139 anti-MMP9 Rabbit Monoclonal antibody reactive to the isotypes of MMP9?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The immunogen of M00139 anti-MMP9 Rabbit Monoclonal antibody is A synthesized peptide derived from human MMP9. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-22
Question
My boss were satisfied with the WB result of your anti-MMP9 Rabbit Monoclonal antibody. However we have observed positive staining in fibrosarcoma extracellular space using this antibody. Is that expected? Could you tell me where is MMP9 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
Based on literature, fibrosarcoma does express MMP9. Generally MMP9 expresses in secreted, extracellular space, extracellular. Regarding which tissues have MMP9 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1480034, 1281792
Fibrosarcoma, Pubmed ID: 1400481, 7669817
Monocytic leukemia, Pubmed ID: 20800577
Neutrophil, Pubmed ID: 1645657, 1464361, 1653055
Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-24
Question
Do you have a BSA free version of anti-MMP9 Rabbit Monoclonal antibody M00139 available?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MMP9 Rabbit Monoclonal antibody M00139 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-14
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tibia using anti-MMP9 Rabbit Monoclonal antibody M00139. Let me know if you need anything else.
D. Jones
Verified customer
Asked: 2019-10-02
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-02
Question
Is a blocking peptide available for product anti-MMP9 Rabbit Monoclonal antibody (M00139)?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
We do provide the blocking peptide for product anti-MMP9 Rabbit Monoclonal antibody (M00139). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-02
Question
Will M00139 anti-MMP9 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
It shows on the product datasheet, M00139 anti-MMP9 Rabbit Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-29
Question
My question regarding product M00139, anti-MMP9 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00139 anti-MMP9 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-27
Question
Will A00306-2 and M00139 be able to work on ELISA experiment?
Verified customer
Asked: 2019-06-17
Answer
The Anti-IFNAR1 Antibody Picoband™ (A00306-2) and Anti-MMP9 Rabbit Monoclonal Antibody (M00139 are not experimentally validated for the ELISA experiment. Please run a pilot test if you want to try.
Boster Scientific Support
Answered: 2019-06-17
Question
We are currently using anti-MMP9 Rabbit Monoclonal antibody M00139 for rat tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
The anti-MMP9 Rabbit Monoclonal antibody (M00139) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-17
Question
Will anti-MMP9 Rabbit Monoclonal antibody M00139 work for IHC with tibia?
Verified Customer
Verified customer
Asked: 2019-05-02
Answer
According to the expression profile of tibia, MMP9 is highly expressed in tibia. So, it is likely that anti-MMP9 Rabbit Monoclonal antibody M00139 will work for IHC with tibia.
Boster Scientific Support
Answered: 2019-05-02
Question
See attached the WB image, lot number and protocol we used for tibia using anti-MMP9 Rabbit Monoclonal antibody M00139. Please let me know if you require anything else.
M. Brown
Verified customer
Asked: 2019-02-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-13
Question
My lab would like using your anti-MMP9 Rabbit Monoclonal antibody for cytokine-mediated signaling pathway studies. Has this antibody been tested with western blotting on kidney tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-01-09
Answer
Thank you for your inquiry. This M00139 anti-MMP9 Rabbit Monoclonal antibody is validated on tissue lysate, kidney tissue, lung tissue. It is guaranteed to work for IF, IHC, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-01-09
Question
We purchased anti-MMP9 Rabbit Monoclonal antibody for IF on fibrosarcoma last year. I am using human, and I plan to use the antibody for IHC next. We are interested in examining fibrosarcoma as well as umbilical cord blood in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
I looked at the website and datasheets of our anti-MMP9 Rabbit Monoclonal antibody and it seems that M00139 has been tested on human in both IF and IHC. Thus M00139 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-05-26
Question
We have observed staining in rat b-cell. What should we do? Is anti-MMP9 Rabbit Monoclonal antibody supposed to stain b-cell positively?
G. Bhatt
Verified customer
Asked: 2017-02-13
Answer
From what I have seen in literature b-cell does express MMP9. From what I have seen in Uniprot.org, MMP9 is expressed in tibia, umbilical cord blood, b-cell, neutrophil, fibrosarcoma, blood, monocytic leukemia, among other tissues. Regarding which tissues have MMP9 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1480034, 1281792
Fibrosarcoma, Pubmed ID: 1400481, 7669817
Monocytic leukemia, Pubmed ID: 20800577
Neutrophil, Pubmed ID: 1645657, 1464361, 1653055
Umbilical cord blood, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-02-13
Question
I am looking for to test anti-MMP9 Rabbit Monoclonal antibody M00139 on rat tibia for research purposes, then I may be interested in using anti-MMP9 Rabbit Monoclonal antibody M00139 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Li
Verified customer
Asked: 2016-12-26
Answer
The products we sell, including anti-MMP9 Rabbit Monoclonal antibody M00139, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-12-26
Question
I was wanting to use your anti-MMP9 Rabbit Monoclonal antibody for IHC for rat tibia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat tibia identification?
T. Rodriguez
Verified customer
Asked: 2015-09-17
Answer
You can see on the product datasheet, M00139 anti-MMP9 Rabbit Monoclonal antibody has been validated for IF, IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat tibia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-09-17
