Anti-MMP-9 Antibody

Product Name

Anti-MMP-9 Antibody

View all MMP-9 Antibodies

SKU/Catalog Number

A00139-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-MMP-9 Antibody catalog # A00139-2. Tested in WB,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MMP-9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00139-2)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human Presenilin 1 around the phosphorylation site of S357.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00139-2 is reactive to MMP9 in Human, Mouse, Rat

Applications

A00139-2 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

78458 MW

Background of MMP-9

Participates in DNA repair and in chromosomal DNA replication.

Kesti T., J. Biol. Chem. 268:10238-10245(1993).
Syvaeoja J.E.;Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
Asahara H., Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of MMP9 using Anti-MMP-9 Antibody (A00139-2).

ICC staining MMP9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-MMP-9 Antibody A00139-2

Western blot analysis of MMP9 on rat spleen tissue lysate using anti-MMP9 antibody at 1/1

Gene/Protein Information For MMP9 (Source: Uniprot.org, NCBI)

Alternative Names

92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase MMP9 CLG4B, GELB, MANDP2, MMP-9 matrix metallopeptidase 9 matrix metalloproteinase-9|macrophage gelatinase|matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|type V collagenase

For more info on MMP9, check out the MMP9 Infographic

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In this infographic, you will see the following information for MMP9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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