Anti-MLF1 CENPU Antibody

Product Name

Anti-MLF1 CENPU Antibody

View all Cenpu Antibodies

SKU/Catalog Number

A07180

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-MLF1 CENPU Antibody (Catalog # A07180). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-MLF1 CENPU Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07180)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a 200 residue recombinant protein corresponding to the amino terminal end of human MLF1IP protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07180 is reactive to Cenpu in Human

Applications

A07180 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

47522 MW

Background of Cenpu

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:1,400,000
IHC: User optimized
WB: 1:500- 1:2,000

Validation Images & Assay Conditions

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Western blot analysis of MLF1 expression in detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (-lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. MLF1 was detected using rabbit anti-MLF1 Antigen Affinity purified polyclonal antibody (Catalog # A07180) at 1:1000. Personal Communication, K.S. Lee, NCI, Bethesda, MD.

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MLF1 was detected in paraffin-embedded sections of human cerebellum tissues using rabbit anti-MLF1 Antigen Affinity purified polyclonal antibody (Catalog # A07180) at 5μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).

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MLF1 was detected in paraffin-embedded sections of human heart tissues using rabbit anti-MLF1 Antigen Affinity purified polyclonal antibody (Catalog # A07180) at 5μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).

Gene/Protein Information For Cenpu (Source: Uniprot.org, NCBI)

Alternative Names

Centromere protein U Cenpu|1700029A22Rik, Mlf, Mlf1ip|centromere protein U|centromere protein U|CENP-U|MLF1-interacting protein|myeloid leukemia factor 1 interacting protein

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In this infographic, you will see the following information for Cenpu: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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