Click image to see more details
Product Info Summary
| SKU: | A07180 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MLF1 CENPU Antibody
SKU/Catalog Number
A07180
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-MLF1 CENPU Antibody (Catalog # A07180). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-MLF1 CENPU Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07180)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a 200 residue recombinant protein corresponding to the amino terminal end of human MLF1IP protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07180 is reactive to CENPU in Human
Reconstitution
Calculated molecular weight
47522 MW
Background of Cenpu
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Myeloid leukemia factor-1 (MLF1) Interacting Protein (also known as PBIP1, MLF1IP1, KLIP1 or KSHV latent nuclear antigen interacting protein 1) is a novel polo-like kinase 1 (Plk1) substrate. Plk1 phosphorylation of MLF1IP induces ubiquitination and degradation of MLF1IP prior to the metaphase/anaphase transition. Several Plk1-dependent phosphorylation sites have been identified on MLF1IP by mass spectrometry. Mutations of these sites stabilize MLF1IP and inhibit mitotic progression. Subsequent in vitro and in vivo MLF1IP phosphorylation and stability assays have revealed that phosphorylation of Thr78 is critical for triggering Plk1-dependent MLF1IP degradation. Expression of a non-degradable Thr78Ala mutant was sufficient to induce a mitotic block. Timely phosphorylation of MLF1IP on Thr78 by Plk1 is critical for eliminating the MLF1IP-imposed mitotic block prior to anaphase onset. MLF1IP is speculated to be a novel tumor suppressor, whose function is required for proper sister-chromatid separation. Loss of MLF1IP function may result in improper segregation of chromosomes and genomic instability, thus promoting tumorigenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07180 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:1,400,000
IHC: User optimized
WB: 1:500- 1:2,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of MLF1 expression in detection of endogenous MLF1IP protein (a tier of four modified protein bands indicated by the arrowheads) in lysates of Hela cells (-lane). Cells treated with MLF1IP / PBIP1 shRNA (+ lane) show no staining. MLF1 was detected using rabbit anti-MLF1 Antigen Affinity purified polyclonal antibody (Catalog # A07180) at 1:1000. Personal Communication, K.S. Lee, NCI, Bethesda, MD.
Click image to see more details
MLF1 was detected in paraffin-embedded sections of human cerebellum tissues using rabbit anti-MLF1 Antigen Affinity purified polyclonal antibody (Catalog # A07180) at 5μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Click image to see more details
MLF1 was detected in paraffin-embedded sections of human heart tissues using rabbit anti-MLF1 Antigen Affinity purified polyclonal antibody (Catalog # A07180) at 5μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Protein Target Info & Infographic
Gene/Protein Information For CENPU (Source: Uniprot.org, NCBI)
Gene Name
CENPU
Full Name
Centromere protein U
Weight
47522 MW
Superfamily
CENP-U/AME1 family
Alternative Names
MLF1, MLF1 Interacting Protein, Centromere protein U, CENP-U, Centromere protein of 50 kDa, CENP-50, Interphase centromere complex protein 24, KSHV latent nuclear antigen-interacting protein 1, MLF1-interacting protein, Polo-box-interacting protein 1, ICEN24, PBIP1, KLIP1 Cenpu|1700029A22Rik, Mlf, Mlf1ip|centromere protein U|centromere protein U|CENP-U|MLF1-interacting protein|myeloid leukemia factor 1 interacting protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CENPU, check out the CENPU Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CENPU: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MLF1 CENPU Antibody (A07180)
Hello CJ!
No publications found for A07180
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MLF1 CENPU Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-MLF1 CENPU Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-MLF1 CENPU Antibody
Question
We are currently using anti-MLF1 antibody A07180 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
The anti-MLF1 antibody (A07180) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-27
