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Product Info Summary
| SKU: | A05807 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-MKRN3 Antibody
SKU/Catalog Number
A05807
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-MKRN3 Antibody (Catalog # A05807). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
MKRN3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-MKRN3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05807)
Host
Rabbit
Contents
MKRN3 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MKRN3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human MKRN3. The immunogen is located within amino acids 400 - 450 of MKRN3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
MKRN3 antibody is human specific. MKRN3 antibody is predicted to not cross-react with other members of the MKRN protein family.
Reactive Species
A05807 is reactive to MKRN3 in Human, Mouse, Rat
Applications
A05807 is guaranteed for ELISA, IF, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
68834 MW
Background of MKRN3
The Makorin ring finger 3 (MKRN3) protein contains a RING (C3HC4) zinc finger motif and several C3H zinc finger motifs. The MKRN3 gene is intronless and imprinted, with expression only from the paternal allele. Disruption of the imprinting at this locus may contribute to Prader-Willi syndrome (1), but a deletion of the gene does not (2). A deficiency of MKRN3 has been shown to cause central precocious puberty in humans (3).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MKRN3 antibody can be used for detection of MKRN3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MKRN3 in human spleen tissue lysate with MKRN3 antibody at (A)1 and (B) 2 μg/ml.
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Immunofluorescence of MKRN3 in human spleen tissue with MKRN3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For MKRN3 (Source: Uniprot.org, NCBI)
Gene Name
MKRN3
Full Name
Probable E3 ubiquitin-protein ligase makorin-3
Weight
68834 MW
Alternative Names
EC 6.3.2.-; makorin ring finger protein 3; MGC88288; RNF63D15S9; ZFP127RING finger protein 63; Zinc finger protein 127; ZNF127probable E3 ubiquitin-protein ligase makorin-3 MKRN3 CPPB2, D15S9, RNF63, ZFP127, ZNF127 makorin ring finger protein 3 probable E3 ubiquitin-protein ligase makorin-3|RING finger protein 63|RING-type E3 ubiquitin transferase makorin-3|zinc finger protein 127
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MKRN3, check out the MKRN3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MKRN3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MKRN3 Antibody (A05807)
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