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Product Info Summary
| SKU: | M10562 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-MKRN2 Antibody
SKU/Catalog Number
M10562
Size
50 µl
Description
Boster Bio Anti-MKRN2 Antibody (Catalog # M10562). Tested in WB, Flow Cytometry, IHC-P, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MKRN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10562)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1556CT631.230.55.49
Isotype
IgG1,k
Immunogen
This MKRN2 antibody is generated from a mouse immunized with recombinant protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M10562 is reactive to MKRN2 in Human
Applications
M10562 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
46940 Da
Background of MKRN2
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:4000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling MKRN2 with M10562 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on U-2 OS cell line. The nuclear counter stain is DAPI (blue).
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All lanes : Anti-MKRN2 Antibody at 1:4000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 47 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M10562 staining MKRN2 in human heart tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Overlay histogram showing K562 cells stained with M10562 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (M10562, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Protein Target Info & Infographic
Gene/Protein Information For MKRN2 (Source: Uniprot.org, NCBI)
Gene Name
MKRN2
Full Name
Probable E3 ubiquitin-protein ligase makorin-2
Weight
46940 Da
Alternative Names
EC 6.3.2.-; HSPC070; makorin ring finger protein 2; probable E3 ubiquitin-protein ligase makorin-2; RNF62RING finger protein 62 MKRN2 HSPC070, RNF62 makorin ring finger protein 2 probable E3 ubiquitin-protein ligase makorin-2|RING finger protein 62|RING-type E3 ubiquitin transferase makorin-2|makorin RING zinc-finger protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MKRN2, check out the MKRN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MKRN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MKRN2 Antibody (M10562)
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