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Product Info Summary
| SKU: | A04885 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Mitochondrial chaperone BCS1 BCS1L Antibody
SKU/Catalog Number
A04885
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Mitochondrial chaperone BCS1 BCS1L Antibody catalog # A04885. Tested in WB,IHC,ICC/IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mitochondrial chaperone BCS1 BCS1L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04885)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant fusion protein of human BCS1L(NP_004319.1).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04885 is reactive to BCS1L in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
47534 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04885 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC/IF: 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of extracts of various cell lines, using BCS1L antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST
Detection: ECL Basic Kit .
Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human stomach using BCS1L antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Immunohistochemistry of paraffin-embedded rat brain using BCS1L antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Immunofluorescence analysis of U2OS cells using BCS1L antibody at dilution of 1:100. Blue: DAPI for nuclear staining
Protein Target Info & Infographic
Gene/Protein Information For BCS1L (Source: Uniprot.org, NCBI)
Gene Name
BCS1L
Full Name
Mitochondrial chaperone BCS1
Weight
47534 MW
Superfamily
AAA ATPase family
Alternative Names
Mitochondrial chaperone BCS1;h-BCS1;BCS1-like protein;BCS1L;BCS1; BCS1L BCS, BCS1, BJS, FLNMS, GRACILE, Hs.6719, MC3DN1, PTD, h-BCS, h-BCS1 BCS1 homolog, ubiquinol-cytochrome c reductase complex chaperone mitochondrial chaperone BCS1|BC1 (ubiquinol-cytochrome c reductase) synthesis-like|BCS1-like protein|mitochondrial complex III assembly
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCS1L, check out the BCS1L Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCS1L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mitochondrial chaperone BCS1 BCS1L Antibody (A04885)
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