Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | PB9274 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MIF Antibody Picoband®
SKU/Catalog Number
PB9274
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MIF Antibody Picoband® catalog # PB9274. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MIF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9274)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MIF recombinant protein (Position: P2-A115). Human MIF shares 89% and 90% amino acid (aa) sequence identity with mouse and rat MIF respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9274 is reactive to MIF in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
12 kDa
Calculated molecular weight
12476 MW
Background of MIF
Macrophage migration inhibitory factor (MIF or MMIF), also known as GIF, is a protein that in humans is encoded by the MIF gene. It is a cytokine released by T-lymphocytes, macrophages, and the pituitary gland that serves to integrate peripheral and central inflammatory responses. MIF gene has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb. The localization of the human gene for MIF is to chromosome 22q11.2. MIF plays a critical role in inflammatory diseases and atherogenesis. It is also involved in cell-mediated immunity and immunoregulation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9274 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Jurkat whole cell, human K562 whole cell, human Hela whole cell, human HepG2 whole cell
IHC: human liver cancer tissue, human lung cancer tissue
ICC/IF: Hela cell
FCM: Jurkat cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of MIF using anti-MIF antibody (PB9274).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MIF antigen affinity purified polyclonal antibody (Catalog # PB9274) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MIF at approximately 12 kDa. The expected band size for MIF is at 12 kDa.
Click image to see more details
Figure 2. IHC analysis of MIF using anti-MIF antibody (PB9274).
MIF was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MIF Antibody (PB9274) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of MIF using anti-MIF antibody (PB9274).
MIF was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-MIF Antibody (PB9274) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of MIF using anti-MIF antibody (PB9274).
MIF was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-MIF Antibody (PB9274) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Flow Cytometry analysis of Jurkat cells using anti-MIF antibody (PB9274).
Overlay histogram showing Jurkat cells stained with PB9274 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MIF Antibody (PB9274, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MIF (Source: Uniprot.org, NCBI)
Gene Name
MIF
Full Name
Macrophage migration inhibitory factor
Weight
12476 MW
Superfamily
MIF family
Alternative Names
Macrophage migration inhibitory factor;MIF;5.3.2.1;Glycosylation-inhibiting factor;GIF;L-dopachrome isomerase;L-dopachrome tautomerase;5.3.3.12;Phenylpyruvate tautomerase;MIF;GLIF, MMIF; MIF GIF, GLIF, MMIF macrophage migration inhibitory factor macrophage migration inhibitory factor|L-dopachrome isomerase|L-dopachrome tautomerase|epididymis secretory sperm binding protein|macrophage migration inhibitory factor (glycosylation-inhibiting factor)|phenylpyruvate tautomerase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MIF, check out the MIF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MIF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MIF Antibody Picoband® (PB9274)
Hello CJ!
No publications found for PB9274
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MIF Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-MIF Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-MIF Antibody Picoband®
Question
Our lab want to know about to test anti-MIF antibody PB9274 on human platelet for research purposes, then I may be interested in using anti-MIF antibody PB9274 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Gonzalez
Verified customer
Asked: 2020-04-22
Answer
The products we sell, including anti-MIF antibody PB9274, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-22
Question
My colleagues were content with the WB result of your anti-MIF antibody. However we have observed positive staining in skin uterus secreted using this antibody. Is that expected? Could you tell me where is MIF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
Based on literature, skin uterus does express MIF. Generally MIF expresses in secreted. Regarding which tissues have MIF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, Lung, Skin, and Uterus, Pubmed ID: 15489334
Lens, Pubmed ID: 7679497
Liver, Pubmed ID: 1286669, 24275569
Platelet, Pubmed ID: 12665801
Boster Scientific Support
Answered: 2020-03-23
Question
I am looking for using your anti-MIF antibody for positive regulation of map kinase activity studies. Has this antibody been tested with western blotting on hut whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
Thanks for your inquiry. This PB9274 anti-MIF antibody is validated on u87 whole cell lysate, jurkat whole cell lysate, hut whole cell lysate, a549 whole cell lysate, hepg2 whole cell lysate, human tonsil tissue. It is guaranteed to work for IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-02-20
Question
We are currently using anti-MIF antibody PB9274 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
The anti-MIF antibody (PB9274) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-07
Question
Will PB9274 anti-MIF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
You can see on the product datasheet, PB9274 anti-MIF antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-29
Question
I was wanting to use your anti-MIF antibody for ICC for human platelet on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human platelet identification?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
You can see on the product datasheet, PB9274 anti-MIF antibody has been validated for IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human platelet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-06
Question
Is a blocking peptide available for product anti-MIF antibody (PB9274)?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
We do provide the blocking peptide for product anti-MIF antibody (PB9274). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-27
Question
We have been able to see staining in human brain. What should we do? Is anti-MIF antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
According to literature brain does express MIF. According to Uniprot.org, MIF is expressed in left adrenal gland, brain, brain, lung, skin uterus, platelet, liver, lens, among other tissues. Regarding which tissues have MIF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Brain, Lung, Skin, and Uterus, Pubmed ID: 15489334
Lens, Pubmed ID: 7679497
Liver, Pubmed ID: 1286669, 24275569
Platelet, Pubmed ID: 12665801
Boster Scientific Support
Answered: 2019-08-19
Question
I see that the anti-MIF antibody PB9274 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-31
Question
My question regarding product PB9274, anti-MIF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9274 anti-MIF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-14
Question
Is this PB9274 anti-MIF antibody reactive to the isotypes of MIF?
Verified Customer
Verified customer
Asked: 2019-03-11
Answer
The immunogen of PB9274 anti-MIF antibody is E.coli-derived human MIF recombinant protein (Position: P2-A115). Human MIF shares 89% and 90% amino acid (aa) sequence identity with mouse and rat MIF respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-03-11
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-MIF antibody PB9274. Let me know if you need anything else.
W. Dhar
Verified customer
Asked: 2018-10-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-18
Question
Does anti-MIF antibody PB9274 work for ICC with platelet?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
According to the expression profile of platelet, MIF is highly expressed in platelet. So, it is likely that anti-MIF antibody PB9274 will work for ICC with platelet.
Boster Scientific Support
Answered: 2018-07-11
Question
Do you have a BSA free version of anti-MIF antibody PB9274 available?
Verified Customer
Verified customer
Asked: 2017-11-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-MIF antibody PB9274 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-13
Question
Here is the WB image, lot number and protocol we used for platelet using anti-MIF antibody PB9274. Please let me know if you require anything else.
S. Evans
Verified customer
Asked: 2015-02-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-02-13
Question
Our lab used your anti-MIF antibody for WB on liver in the past. I am using human, and I plan to use the antibody for ICC next. We are interested in examining liver as well as left adrenal gland in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
S. Taylor
Verified customer
Asked: 2013-07-01
Answer
I viewed the website and datasheets of our anti-MIF antibody and it seems that PB9274 has been validated on human in both WB and ICC. Thus PB9274 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-07-01
