Product Info Summary
SKU: | A08089 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-MICAL3 Antibody Picoband™
SKU/Catalog Number
A08089
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MICAL3 Antibody Picoband™ catalog # A08089. Tested in WB, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MICAL3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08089)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human MICAL3 recombinant protein (Position: K458-L1592). Human MICAL3 shares 81.9% amino acid (aa) sequence identity with mouse MICAL3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08089 is reactive to MICAL3 in Human, Mouse, Rat
Applications
A08089 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
224-280 kDa
Calculated molecular weight
Background of MICAL3
Microtubule-associated monoxygenase, calponin and LIM domain containing 3, also known as MICAL3, is a human gene. MICAL3 (microtubule associated monoxygenase, calponin and LIM domain containing 3), also known as KIAA1364, is a 976 amino acid protein that localizes to both the cytoplasm and the cytoskeleton and contains one LIM zinc-binding domain and one calponin-homology domain. Expressed ubiquitously, MICAL3 uses FAD as a cofactor to interact with Rab 1B and, via this interaction, is thought to play a role in spermatid development. MICAL3 exists as two alternatively spliced isoforms that are encoded by a gene which maps to human chromosome 22q11.21. Chromosome 22 houses over 500 genes, some of which are involved in Phelan-McDermid syndrome, schizophrenia and Neurofibromatosis type 2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
![a08089 mical3 primary antibodies wb testing 1 a08089 mical3 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a08089-mical3-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of MICAL3 using anti-MICAL3 antibody (A08089).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human Hacat whole cell lysates,
Lane 5: rat testis tissue lysates,
Lane 6: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MICAL3 antigen affinity purified polyclonal antibody (Catalog # A08089) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MICAL3 at approximately 224-280 kDa. The expected band size for MICAL3 is at 224 kDa.
![a08089 mical3 primary antibodies fcm testing 2 a08089 mical3 primary antibodies fcm testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a08089-mical3-primary-antibodies-fcm-testing-2.png)
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Figure 2. Flow Cytometry analysis of U251 cells using anti-MICAL3 antibody (A08089).
Overlay histogram showing U251 cells stained with A08089 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MICAL3 Antibody (A08089, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For MICAL3 (Source: Uniprot.org, NCBI)
Gene Name
MICAL3
Full Name
[F-actin]-monooxygenase MICAL3
Weight
Superfamily
Mical family
Alternative Names
microtubule associated monoxygenase, calponin and LIM domain containing 3 MICAL3 MICAL-3 microtubule associated monooxygenase, calponin and LIM domain containing 3 [F-actin]-monooxygenase MICAL3|[F-actin]-methionine sulfoxide oxidase MICAL3|flavoprotein oxidoreductase MICAL3|molecule interacting with CasL protein 3|protein MICAL-3|protein-methionine sulfoxide oxidase MICAL3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MICAL3, check out the MICAL3 Infographic
![MICAL3 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MICAL3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MICAL3 Antibody Picoband™ (A08089)
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