Anti-Mib1/Mindbomb Antibody Picoband®

Product Name

Anti-Mib1/Mindbomb Antibody Picoband®

View all Mind Bomb 1/MIB1 Antibodies

SKU/Catalog Number

A01387-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Mib1/Mindbomb Antibody Picoband® catalog # A01387-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Mib1/Mindbomb Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01387-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Mib1 Mindbomb recombinant protein (Position: H465-E732).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01387-1 is reactive to MIB1 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

110 kDa

Calculated molecular weight

33899 MW

Background of Mind Bomb 1/MIB1

E3 ubiquitin-protein ligase MIB1 is an enzyme that in humans is encoded by the MIB1 gene. It is mapped to 18q11.2. This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01387-1 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml

Positive Control

WB: human U87 whole cell, rat liver tissue, rat brain tissue, mouse liver tissue, mouse brain tissue

Validation Images & Assay Conditions

Gene/Protein Information For MIB1 (Source: Uniprot.org, NCBI)

Alternative Names

E3 ubiquitin-protein ligase MIB1; DAPK-interacting protein 1; DIP-1; Mind bomb homolog 1; RING-type E3 ubiquitin transferase MIB1 ; Zinc finger ZZ type with ankyrin repeat domain protein 2; MIB1; DIP1; KIAA1323; ZZANK2 MIB1 DIP-1, DIP1, LVNC7, MIB, ZZANK2, ZZZ6 MIB E3 ubiquitin protein ligase 1 E3 ubiquitin-protein ligase MIB1|DAPK-interacting protein 1|RING-type E3 ubiquitin transferase MIB1|mindbomb E3 ubiquitin protein ligase 1|ubiquitin ligase mind bomb|zinc finger ZZ type with ankyrin repeat domain protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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