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Product Info Summary
| SKU: | A03200 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Bovine, Human, Mouse, Pig, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Mi2- alpha Monoclonal Antibody
SKU/Catalog Number
A03200
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Mi2- alpha Monoclonal Antibody catalog # A03200. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Bovine, Human, Mouse, Pig, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Mi2- alpha Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03200)
Host
Mouse
Contents
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Clonality
Monoclonal
Clone Number
1A4
Isotype
IgG
Immunogen
Purified recombinant human Mi2-α (C-terminus) protein fragments expressed in E.coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03200 is reactive to CHD3 in Bovine, Human, Mouse, Pig, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
226592 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03200 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-1:2000
IHC, 1:500-1:1000
IF, 1:100-1:500
FC, 1:100-1:200
Validation Images & Assay Conditions
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Western Blot (WB) analysis using Mi2-alpha Monoclonal antibody against Y7P, Raji cell lysate.
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Immunofluorescence (IF) analysis of HeLa cells using Mi2-alpha Monoclonal antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Colon using Mi2-alpha Monoclonal antibody.
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Flow cytometric (FCM) analysis of K562 cells stained with Mi2-alpha Monoclonal antibody (red), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
Protein Target Info & Infographic
Gene/Protein Information For CHD3 (Source: Uniprot.org, NCBI)
Gene Name
CHD3
Full Name
Chromodomain-helicase-DNA-binding protein 3
Weight
226592 MW
Superfamily
SNF2/RAD54 helicase family
Alternative Names
Chromodomain-helicase-DNA-binding protein 3;CHD-3;3.6.4.12;ATP-dependent helicase CHD3;Mi-2 autoantigen 240 kDa protein;Mi2-alpha;Zinc finger helicase;hZFH;CHD3; CHD3 Mi-2a, Mi2-ALPHA, SNIBCPS, ZFH chromodomain helicase DNA binding protein 3 chromodomain-helicase-DNA-binding protein 3|ATP-dependent helicase CHD3|CHD-3|hZFH|mi-2 auto 240 kDa protein|zinc finger helicase|zinc-finger helicase (Snf2-like)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHD3, check out the CHD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mi2- alpha Monoclonal Antibody (A03200)
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No publications found for A03200
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Customer Q&As
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3 Customer Q&As for Anti-Mi2- alpha Monoclonal Antibody
Question
I have attached the WB image, lot number and protocol we used for thymus using anti-Mi2- alpha Monoclonal antibody A03200. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-11
Question
We are currently using anti-Mi2- alpha Monoclonal antibody A03200 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says bovine, human, mouse, pig, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
The anti-Mi2- alpha Monoclonal antibody (A03200) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-26
Question
Is there a BSA free version of anti-Mi2- alpha Monoclonal antibody A03200 available?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Mi2- alpha Monoclonal antibody A03200 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-06-19
