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Product Info Summary
| SKU: | A01533 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-CHD7 Antibody
SKU/Catalog Number
A01533
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CHD7 Antibody (Catalog # A01533). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
CHD7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CHD7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01533)
Host
Rabbit
Contents
CHD7 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CHD7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CHD7. The immunogen is located within amino acids 670 - 720 of CHD7.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Multiple isoforms of CHD7 are known to exist.
Reactive Species
A01533 is reactive to CHD7 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
335927 MW
Background of CHD7
CHD proteins belong to a superfamily of proteins of ATP-dependent chromatin remodeling enzymes that have a unique combination of functional domains, including two N-terminal chromodomains, a SNF2-like ATPase/helicase domain and a DNA-binding domain. These proteins are thought to play a role in early embryonic development by affecting chromatin structure and gene expression. Mutations in one member of this family, CHD7, result in CHARGE syndrome. It colocalizes with embryonic stem (ES) cell master regulators OCT4/POU5F1, SOX2, and NANOG and is thought to modulate ES-specific gene transcription. Together with SOX2, CHD7 has been suggested to also regulate several human disease genes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01533 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CHD7 antibody can be used for detection of CHD7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CHD7 in SK-N-SH cell lysate with CHD7 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of CHD7 in mouse brain tissue with CHD7 antibody at 5 μg/mL.
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Immunofluorescence of CHD7 in mouse brain tissue with CHD7 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CHD7 (Source: Uniprot.org, NCBI)
Gene Name
CHD7
Full Name
Chromodomain-helicase-DNA-binding protein 7
Weight
335927 MW
Superfamily
SNF2/RAD54 helicase family
Alternative Names
CRG, HH5, IS3, KAL5, KIAA1416, Chromodomain-helicase-DNA-binding protein 7, ATP-dependent helicase CHD7, CHD-7 CHD7 CRG, HH5, IS3, KAL5 chromodomain helicase DNA binding protein 7 chromodomain-helicase-DNA-binding protein 7|ATP-dependent helicase CHD7|CHARGE association
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHD7, check out the CHD7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHD7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CHD7 Antibody (A01533)
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