Product Info Summary
SKU: | M06123 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-mGluR2 GRM2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M06123
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-mGluR2 GRM2 Rabbit Monoclonal Antibody catalog # M06123. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-mGluR2 GRM2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06123)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IEI-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human mGluR2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06123 is reactive to GRM2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
25 kDa
Calculated molecular weight
95568 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06123 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of mGluR2 expression in Mouse brain lysate.
Click image to see more details
All lanes use the Antibody at 1:1W dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For GRM2 (Source: Uniprot.org, NCBI)
Gene Name
GRM2
Full Name
Metabotropic glutamate receptor 2
Weight
95568 MW
Superfamily
G-protein coupled receptor 3 family
Alternative Names
Metabotropic glutamate receptor 2;mGluR2;GRM2;GPRC1B, MGLUR2; GRM2 GLUR2, GPRC1B, MGLUR2, mGlu2 glutamate metabotropic receptor 2 metabotropic glutamate receptor 2|glutamate receptor homolog|glutamate receptor, metabotropic 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GRM2, check out the GRM2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GRM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-mGluR2 GRM2 Rabbit Monoclonal Antibody (M06123)
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No publications found for M06123
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Customer Q&As
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4 Customer Q&As for Anti-mGluR2 GRM2 Rabbit Monoclonal Antibody
Question
I see that the anti-mGluR2 Rabbit Monoclonal antibody M06123 works with IF, what is the protocol used to produce the result images on the product page?
D. Taylor
Verified customer
Asked: 2020-02-20
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-20
Question
Would M06123 anti-mGluR2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
It shows on the product datasheet, M06123 anti-mGluR2 Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-10
Question
My question regarding product M06123, anti-mGluR2 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M06123 anti-mGluR2 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-26
Question
Please see the WB image, lot number and protocol we used for brain using anti-mGluR2 Rabbit Monoclonal antibody M06123. Please let me know if you require anything else.
W. Rodriguez
Verified customer
Asked: 2017-12-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-12