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Product Info Summary
| SKU: | A04891 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Vesicular Acetylcholine Transporter/SLC18A3 Antibody Picoband®
View all VAChT/SLC18A3 Antibodies
SKU/Catalog Number
A04891
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Vesicular Acetylcholine Transporter/SLC18A3 Antibody Picoband® catalog # A04891. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vesicular Acetylcholine Transporter/SLC18A3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04891)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SLC18A3, different from the related mouse and rat sequences by five amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04891 is reactive to SLC18A3 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
56903 MW
Background of VAChT/SLC18A3
The Vesicular acetylcholine transporter (VAChT), also known as SLC18A3, is a neurotransmitter transporter which is responsible for loadingacetylcholine (ACh) into secretory organelles in neurons making acetylcholine available for secretion. It is encoded by Solute carrier family 18, member 3 (SLC18A3) gene. This gene is a member of the vesicular amine transporter family. The encoded transmembrane protein transports acetylcholine into secretory vesicles for release into the extracellular space. Acetylcholine transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04891 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Positive Control
WB: HELA whole cell, HEPA whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SLC18A3 using anti-SLC18A3 antibody (A04891).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: HELA whole cell lysates,
lane 2: HEPA whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC18A3 antigen affinity purified polyclonal antibody (Catalog # A04891) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC18A3 at approximately 55KD. The expected band size for SLC18A3 is at 57KD.
Protein Target Info & Infographic
Gene/Protein Information For SLC18A3 (Source: Uniprot.org, NCBI)
Gene Name
SLC18A3
Full Name
Vesicular acetylcholine transporter
Weight
56903 MW
Superfamily
major facilitator superfamily
Alternative Names
Vesicular acetylcholine transporter;VAChT;Solute carrier family 18 member 3;SLC18A3;VACHT; SLC18A3 CMS21, VACHT solute carrier family 18 member A3 vesicular acetylcholine transporter|solute carrier family 18 (vesicular acetylcholine transporter), member 3|solute carrier family 18 (vesicular acetylcholine), member 3|solute carrier family 18, member 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC18A3, check out the SLC18A3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC18A3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Vesicular Acetylcholine Transporter/SLC18A3 Antibody Picoband® (A04891)
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Customer Q&As
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1 Customer Q&As for Anti-Vesicular Acetylcholine Transporter/SLC18A3 Antibody Picoband®
Question
We are currently using anti-Vesicular Acetylcholine Transporter/SLC18A3 antibody A04891 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
The anti-Vesicular Acetylcholine Transporter/SLC18A3 antibody (A04891) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-21
