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Product Info Summary
| SKU: | A09345-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-MGARP Antibody Picoband®
SKU/Catalog Number
A09345-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-MGARP Antibody Picoband® catalog # A09345-2. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MGARP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09345-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human C4orf49/MGARP recombinant protein (Position: M1-E155). Human C4orf49/MGARP shares 67.3% amino acid (aa) sequence identity with mouse C4orf49/MGARP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09345-2 is reactive to MGARP in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
29.925kDa
Background of OSAP
MGARP is highly enriched in steroidogenic tissues and the visual system. Interestingly, its expression increases as mice develop. Early in development, MGARP is mainly detected in the retina and adrenal gland. At this early developmental stage, its expression is not detectable in the gonads, but its expression in the gonads dramatically increases during the first 2–4 wk after birth. Importantly, MGARP levels correlate with estrogen levels in the ovaries during the estrous cycle, and estrogen regulates the expression of MGARP in a tissue-specific manner and through a feedback regulatory mechanism. Functional inhibition of GnRH with an antagonist strongly reduces MGARP levels, and knockout of leptin (ob/ob) significantly reduces the MGARP expression in follicular granular cells. We proposed a model that elucidates the role MGARP plays in the HPG axis. Within the HPG axis loop, MGARP participates in hormone biosynthesis while being under the regulation of the hormones derived from the HPG axis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09345-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human A2780 whole cell, human A549 whole cell, human SK-OV-3 whole cell, human 293T whole cell, rat brain tissue, rat testis tissue, mouse brain tissue, mouse testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of C4orf49/MGARP using anti-C4orf49/MGARP antibody (A09345-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A2780 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human SK-OV-3 whole cell lysates,
Lane 4: human 293T whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat testis tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C4orf49/MGARP antigen affinity purified polyclonal antibody (Catalog # A09345-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C4orf49/MGARP at approximately 38 kDa. The expected band size for C4orf49/MGARP is at 25 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MGARP (Source: Uniprot.org, NCBI)
Gene Name
MGARP
Full Name
Protein MGARP
Weight
29.925kDa
Alternative Names
chromosome 4 open reading frame 49; hypoxia up-regulated mitochondrial movement regulator; MGC125827; MGC125828; OSAP Mgarp|4930583H14Rik, AI195347, CESP-1, HUMMR, Osap, Qsap|mitochondria localized glutamic acid rich protein|protein MGARP|corneal endothelium-specific protein 1|hypoxia up-regulated mitochondrial movement regulator protein|ovary-specific acidic protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MGARP, check out the MGARP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MGARP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MGARP Antibody Picoband® (A09345-2)
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