Anti-Metalloreductase STEAP1 STEAP1 Antibody

Product Name

Anti-Metalloreductase STEAP1 STEAP1 Antibody

View all STEAP1 Antibodies

SKU/Catalog Number

A07506

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Metalloreductase STEAP1 STEAP1 Antibody (Catalog # A07506). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

STEAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Metalloreductase STEAP1 STEAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07506)

Host

Rabbit

Contents

STEAP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

STEAP1 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human STEAP1. The immunogen is located within the last 50 amino acids of STEAP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This STEAP1 antibody does not cross-react with other STEAP proteins.

Reactive Species

A07506 is reactive to STEAP1 in Human, Mouse, Rat

Applications

A07506 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

39851 MW

Background of STEAP1

The six-transmembrane epithelial antigen of prostate 1 (STEAP1) was the first member of a family of metalloreductases identified as cell-surface antigens in prostate tissue. The normal function of STEAP is still uncertain; unlike other members of the STEAP family, STEAP1 does not promote iron or copper reduction or uptake and lacks the FNO-like reductase domain critical for activity. However, its expression is highly increased in multiple cancer cell lines, including prostate, bladder, colon, and ovarian cancers. Supporting this is evidence that STEAP1 peptides can be used to stimulate CD8+ T cells from healthy donors, enabling them to recognize STEAP1-positive human tumor cells, suggesting that STEAP1 may a potential target for cancer immunotherapy. At least three isoforms of STEAP1 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

STEAP1 antibody can be used for detection of STEAP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of STEAP1 in human spleen tissue lysate with STEAP1 antibody at (A) 1 and (B) 2 μg/mL.

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Immunohistochemistry of STEAP1 in human spleen tissue with STEAP1 antibody at 2.5 μg/mL.

Gene/Protein Information For STEAP1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 1.16.1.-; PRSS24; PRSS24MGC19484; six transmembrane epithelial antigen of the prostate 1; six transmembrane epithelial antigen of the prostate; Six-transmembrane epithelial antigen of prostate 1; STEAP1; STEAPmetalloreductase STEAP1 STEAP1 PRSS24, STEAP STEAP family member 1 metalloreductase STEAP1|STEAP1 metalloreductase|six transmembrane epithelial antigen of the prostate 1|six-transmembrane epithelial antigen of prostate 1

For more info on STEAP1, check out the STEAP1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for STEAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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