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Product Info Summary
| SKU: | PB9186 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Met (c-Met) Antibody Picoband®
View all HGFR/c-MET Antibodies
SKU/Catalog Number
PB9186
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Met (c-Met) Antibody Picoband® catalog # PB9186. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Met (c-Met) Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9186)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Met recombinant protein (Position: D208-S407). Human Met shares 90% and 91% amino acid (aa) sequences identity with mouse and rat Met, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9186 is reactive to MET in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
154 kDa
Calculated molecular weight
155541 MW
Background of HGFR/c-MET
c-Met, also called MET and hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene. It is mapped to 7q31.2. The protein possesses tyrosine kinase activity. MET is a membrane receptor that is essential for embryonic development and wound healing. It induces several biological responses that collectively give rise to a program known as invasive growth. MET is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express MET, which allows these cells to grow invasively in order to generate new tissues in an embryo or regenerate damaged tissues in an adult. However, cancer stem cells are thought to hijack the ability of normal stem cells to express MET, and thus become the cause of cancer persistence and spread to other sites in the body.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9186 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat, Human
Positive Control
WB: rat liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Met (c-Met) using anti-Met (c-Met) antibody (PB9186).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Met (c-Met) antigen affinity purified polyclonal antibody (Catalog # PB9186) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Met (c-Met) at approximately 154 kDa. The expected band size for Met (c-Met) is at 156 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MET (Source: Uniprot.org, NCBI)
Gene Name
MET
Full Name
Hepatocyte growth factor receptor
Weight
155541 MW
Superfamily
protein kinase superfamily
Alternative Names
Hepatocyte growth factor receptor;HGF receptor;2.7.10.1;HGF/SF receptor;Proto-oncogene c-Met;Scatter factor receptor;SF receptor;Tyrosine-protein kinase Met;MET; MET AUTS9, DFNB97, HGFR, RCCP2, c-Met MET proto-oncogene, receptor tyrosine kinase hepatocyte growth factor receptor|HGF receptor|HGF/SF receptor|SF receptor|proto-oncogene c-Met|scatter factor receptor|tyrosine-protein kinase Met
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MET, check out the MET Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MET: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Met (c-Met) Antibody Picoband® (PB9186)
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Customer Q&As
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1 Customer Q&As for Anti-Met (c-Met) Antibody Picoband®
Question
We are currently using anti-Met (c-Met) antibody PB9186 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The anti-Met (c-Met) antibody (PB9186) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-30
