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Product Info Summary
| SKU: | PA1924 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MEKK3/MAP3K3 Antibody Picoband®
SKU/Catalog Number
PA1924
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MEKK3/MAP3K3 Antibody catalog # PA1924. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEKK3/MAP3K3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1924)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MEKK3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1924 is reactive to MAP3K3 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
71 kDa
Calculated molecular weight
70898 MW
Background of MEKK3
MAP3K3 (Mitogen-activated protein kinase kinase kinase 3), also known as MAP/ERK KINASE KINASE 3, MEKK3 or MAPKKK3, is an enzyme that in humans is encoded by the MAP3K3 gene. MAP3K3 is contiguously distal to LYK5 on chromosome 17 (Puffenberger et al., 2007). By yeast 2-hybrid analysis of a mouse T-cell cDNA library, Uhlik et al. (2003) showed that a C-terminal fragment of mouse Osm (CCM2) interacted with Mekk3, which activates p38 in response to sorbitol-induced hyperosmotic conditions. Mekk3 and Osm colocalized in the cytoplasmic compartment of cotransfected cells, and the Mekk3-Osm complex was recruited to Rac1 and cytoskeletal actin-containing membrane ruffles in response to sorbitol treatment. Protein interaction assays showed that Osm interacted directly with the Mekk3 substrate Mkk3 (MAP2K3), with actin, and with both GDP- and GTP-loaded Rac1. Uhlik et al. (2003) concluded that the RAC1-OSM-MEKK3-MKK3 complex is required for regulation of p38 activity in response to osmotic shock.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1924 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human Daudi whole cell, human A431 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MEKK3/MAP3K3 using anti-MEKK3/MAP3K3 antibody (PA1924).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEKK3/MAP3K3 antigen affinity purified polyclonal antibody (Catalog # PA1924) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEKK3/MAP3K3 at approximately 71 kDa. The expected band size for MEKK3/MAP3K3 is at 71 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MAP3K3 (Source: Uniprot.org, NCBI)
Gene Name
MAP3K3
Full Name
Mitogen-activated protein kinase kinase kinase 3
Weight
70898 MW
Superfamily
protein kinase superfamily
Alternative Names
Mitogen-activated protein kinase kinase kinase 3;2.7.11.25;MAPK/ERK kinase kinase 3;MEK kinase 3;MEKK 3;MAP3K3;MAPKKK3, MEKK3; MAP3K3 MAPKKK3, MEKK3 mitogen-activated protein kinase kinase kinase 3 mitogen-activated protein kinase kinase kinase 3|MAP/ERK kinase kinase 3|MAPK/ERK kinase kinase 3|MEK kinase 3|MEKK 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP3K3, check out the MAP3K3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP3K3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEKK3/MAP3K3 Antibody Picoband® (PA1924)
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No publications found for PA1924
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Customer Q&As
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7 Customer Q&As for Anti-MEKK3/MAP3K3 Antibody Picoband®
Question
My question regards to test anti-MEKK3/MAP3K3 antibody PA1924 on human cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-MEKK3/MAP3K3 antibody PA1924 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
The products we sell, including anti-MEKK3/MAP3K3 antibody PA1924, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-21
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-MEKK3/MAP3K3 antibody PA1924. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-20
Question
Is this PA1924 anti-MEKK3/MAP3K3 antibody reactive to the isotypes of MAP3K3?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
The immunogen of PA1924 anti-MEKK3/MAP3K3 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human MEKK3(11-25aa MNDLVALQMNRRHRM). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-31
Question
I see that the anti-MEKK3/MAP3K3 antibody PA1924 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-06
Question
I was wanting to use your anti-MEKK3/MAP3K3 antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2018-12-24
Answer
It shows on the product datasheet, PA1924 anti-MEKK3/MAP3K3 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-24
Question
I have a question about product PA1924, anti-MEKK3/MAP3K3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1924 anti-MEKK3/MAP3K3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-23
Question
Is a blocking peptide available for product anti-MEKK3/MAP3K3 antibody (PA1924)?
R. Miller
Verified customer
Asked: 2017-03-15
Answer
We do provide the blocking peptide for product anti-MEKK3/MAP3K3 antibody (PA1924). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-03-15
