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Product Info Summary
| SKU: | M00996 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MEK2 MAP2K2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00996
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MEK2 MAP2K2 Rabbit Monoclonal Antibody catalog # M00996. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEK2 MAP2K2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00996)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HBI-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MEK2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00996 is reactive to MAP2K2 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
42 kDa
Calculated molecular weight
44424 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00996 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of MEK2 expression in Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MAP2K2 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K2
Full Name
Dual specificity mitogen-activated protein kinase kinase 2
Weight
44424 MW
Superfamily
protein kinase superfamily
Alternative Names
Dual specificity mitogen-activated protein kinase kinase 2;MAP kinase kinase 2;MAPKK 2;2.7.12.2;ERK activator kinase 2;MAPK/ERK kinase 2;MEK 2;MAP2K2;MEK2, MKK2, PRKMK2; MAP2K2 CFC4, MAPKK2, MEK2, MKK2, PRKMK2 mitogen-activated protein kinase kinase 2 dual specificity mitogen-activated protein kinase kinase 2|ERK activator kinase 2|MAP kinase kinase 2|MAPK/ERK kinase 2|mitogen-activated protein kinase kinase 2, p45
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K2, check out the MAP2K2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEK2 MAP2K2 Rabbit Monoclonal Antibody (M00996)
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No publications found for M00996
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Customer Q&As
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3 Customer Q&As for Anti-MEK2 MAP2K2 Rabbit Monoclonal Antibody
Question
Is there a BSA free version of anti-MEK2 Rabbit Monoclonal antibody M00996 available?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MEK2 Rabbit Monoclonal antibody M00996 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-17
Question
Will anti-MEK2 Rabbit Monoclonal antibody M00996 work on bovine IF with testis?
M. Taylor
Verified customer
Asked: 2018-08-03
Answer
Our lab technicians have not tested anti-MEK2 Rabbit Monoclonal antibody M00996 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-MEK2 Rabbit Monoclonal antibody M00996 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine testis in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-03
Question
Will anti-MEK2 Rabbit Monoclonal antibody M00996 work for IF with testis?
D. Brown
Verified customer
Asked: 2016-03-01
Answer
According to the expression profile of testis, MAP2K2 is highly expressed in testis. So, it is likely that anti-MEK2 Rabbit Monoclonal antibody M00996 will work for IF with testis.
Boster Scientific Support
Answered: 2016-03-01
