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Product Info Summary
| SKU: | A00292T292 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-MEK1 (phospho-T292) MAP2K1 Antibody
SKU/Catalog Number
A00292T292
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MEK1 (phospho-T292) MAP2K1 Antibody catalog # A00292T292. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEK1 (phospho-T292) MAP2K1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00292T292)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Threonine 292.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00292T292 is reactive to MAP2K1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
43439 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00292T292 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of MAP2K1 using Anti-MEK1 (phospho-T292) MAP2K1 Antibody (A00292T292).
Immunohistochemistry (IHC) analyzes of p-MEK1 (T292) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-MEK1 (phospho-T292) MAP2K1 Antibody A00292T292
Western blot (WB) analysis of p-MEK1 (T292) pAb at 1:500 dilutionLane1:CT26 whole cell lysate(40ug)
Lane2:H9C2 whole cell lysate(40ug)
Lane3:A2780 whole cell lysate(40ug)
Lane4:H1792 whole cell lysate(40ug)
Lane5:SGC7901 whole cell lysate(40ug)
Lane6:A549 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MAP2K1 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K1
Full Name
Dual specificity mitogen-activated protein kinase kinase 1
Weight
43439 MW
Superfamily
protein kinase superfamily
Alternative Names
Dual specificity mitogen-activated protein kinase kinase 1;MAP kinase kinase 1;MAPKK 1;MKK1;2.7.12.2;ERK activator kinase 1;MAPK/ERK kinase 1;MEK 1;MAP2K1;MEK1, PRKMK1; MAP2K1 CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1 mitogen-activated protein kinase kinase 1 dual specificity mitogen-activated protein kinase kinase 1|ERK activator kinase 1|MAPK/ERK kinase 1|MAPKK 1|MEK 1|protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K1, check out the MAP2K1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEK1 (phospho-T292) MAP2K1 Antibody (A00292T292)
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