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Product Info Summary
| SKU: | P00292 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Phospho-MEK1/2 (Ser218,222) MAP2K1 Antibody
SKU/Catalog Number
P00292
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-MEK1/2 (Ser218, 222) MAP2K1 Antibody (Catalog # P00292). Tested in WB applications. This antibody reacts with Mouse, Human.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-MEK1/2 (Ser218,222) MAP2K1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00292)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser218/222 of human MEK1/2, conjugated to keyhole limpet hemocyanin (KLH). Note: The phosphosites in human MEK 1/2 are Ser218 and Ser222; the phosphosites in mouse MEK 1/2 are Ser217 and Ser221. Immunogen species is Human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00292 is reactive to MAP2K1/2 in Human, Mouse
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
43439 MW
Background of MAP2K1
MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in the brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00292 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
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Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45 kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (-) or incubated in the presence of serum (+). Immunolabeling of an additional band at ~95 kDa was also observed.
Protein Target Info & Infographic
Gene/Protein Information For MAP2K1/2 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K1/2
Full Name
Dual specificity mitogen-activated protein kinase kinase 1
Weight
43439 MW
Superfamily
protein kinase superfamily
Alternative Names
AA589381 antibody, CFC3 antibody, Dual specificity mitogen-activated protein kinase kinase 1 antibody, Dual specificity mitogen-activated protein kinase kinase 2 antibody, EC 2.7.12.2 antibody, ERK activator kinase 1 antibody, ERK activator kinase 2 antibody, FLJ26075 antibody, MAP kinase kinase 1 antibody, MAP kinase kinase 2 antibody, MAP2K1 antibody, MAP2K2 antibody, MAPK/ERK kinase 1 antibody, MAPK/ERK kinase 2 antibody, MAPKK 1 antibody, MAPKK1 antibody, MAPKK2 antibody, MEK 1 antibody, MEK1 antibody, MEKK1 antibody, Mitogen activated protein kinase kinase 1 antibody, Mitogen activated protein kinase kinase 2 antibody, Mitogen-activated protein kinase kinase 2, p45 antibody, MK2 antibody, MKK 1 antibody, MKK 2 antibody, MKK1 antibody, MKK2 antibody, MP2K1_HUMAN antibody, PRKMK 1 antibody, PRKMK 2 antibody, Prkmk1 antibody, Prkmk2 antibody, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1) antibody, Protein kinase, mitogen-activated, kinase 1 antibody, Protein kinase, mitogen-activated, kinase 2 antibody MAP2K1 CFC3, MAPKK1, MEK1, MEL, MKK1, PRKMK1 mitogen-activated protein kinase kinase 1 dual specificity mitogen-activated protein kinase kinase 1|ERK activator kinase 1|MAPK/ERK kinase 1|MAPKK 1|MEK 1|protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K1/2, check out the MAP2K1/2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K1/2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-MEK1/2 (Ser218,222) MAP2K1 Antibody (P00292)
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No publications found for P00292
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Customer Q&As
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4 Customer Q&As for Anti-Phospho-MEK1/2 (Ser218,222) MAP2K1 Antibody
Question
I have a question about product P00292, anti-Phospho-MEK1/2 (Ser218,222) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00292 anti-Phospho-MEK1/2 (Ser218,222) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-16
Question
Does anti-Phospho-MEK1/2 (Ser218,222) antibody P00292 work on zebrafish WB with t-cell?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
Our lab technicians have not tested anti-Phospho-MEK1/2 (Ser218,222) antibody P00292 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Phospho-MEK1/2 (Ser218,222) antibody P00292 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-10
Question
Is a blocking peptide available for product anti-Phospho-MEK1/2 (Ser218,222) antibody (P00292)?
Verified Customer
Verified customer
Asked: 2018-02-20
Answer
We do provide the blocking peptide for product anti-Phospho-MEK1/2 (Ser218,222) antibody (P00292). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-02-20
Question
Is this P00292 anti-Phospho-MEK1/2 (Ser218,222) antibody reactive to the isotypes of MAP2K1?
D. Taylor
Verified customer
Asked: 2017-04-05
Answer
The immunogen of P00292 anti-Phospho-MEK1/2 (Ser218,222) antibody is The antigen is a Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser218,222 of human MEK 1/2. Note: The phosphosites in human MEK 1/2 are Ser218 and Ser222; the phosphosites in mouse MEK 1/2 are Ser217 and Ser221. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-04-05
