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Product Info Summary
| SKU: | A15544 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MEGF9/Egfl5 Antibody
SKU/Catalog Number
A15544
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MEGF9/Egfl5 Antibody (Catalog # A15544). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEGF9/Egfl5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15544)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human MEGF9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A15544 is reactive to MEGF9 in Human, Mouse
Reconstitution
Calculated molecular weight
62984 MW
Background of MEGF9
Potassium channel subunit. Modulates channel activity and reduces the ion flow By similarity.
Hirosawa M., DNA Res. 6:329-336(1999).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15544 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from COLO cells, using MEGF9 antibody A15544.
Protein Target Info & Infographic
Gene/Protein Information For MEGF9 (Source: Uniprot.org, NCBI)
Gene Name
MEGF9
Full Name
Multiple epidermal growth factor-like domains protein 9
Weight
62984 MW
Alternative Names
Multiple epidermal growth factor-like domains protein 9;Multiple EGF-like domains protein 9;Epidermal growth factor-like protein 5;EGF-like protein 5;MEGF9;EGFL5, KIAA0818;UNQ671/PRO1305; MEGF9 EGFL5 multiple EGF like domains 9 multiple epidermal growth factor-like domains protein 9|EGF-like-domain, multiple 5|epidermal growth factor-like protein 5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MEGF9, check out the MEGF9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MEGF9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEGF9/Egfl5 Antibody (A15544)
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No publications found for A15544
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-MEGF9/Egfl5 Antibody
Question
Do you have a BSA free version of anti-MEGF9/Egfl5 antibody A15544 available?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MEGF9/Egfl5 antibody A15544 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for trigeminal ganglion using anti-MEGF9/Egfl5 antibody A15544. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-22
Question
See below the WB image, lot number and protocol we used for trigeminal ganglion using anti-MEGF9/Egfl5 antibody A15544. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-11
Question
My question regards to test anti-MEGF9/Egfl5 antibody A15544 on human trigeminal ganglion for research purposes, then I may be interested in using anti-MEGF9/Egfl5 antibody A15544 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
H. Brown
Verified customer
Asked: 2019-05-13
Answer
The products we sell, including anti-MEGF9/Egfl5 antibody A15544, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-13
Question
Would A15544 anti-MEGF9/Egfl5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
T. Roberts
Verified customer
Asked: 2017-03-22
Answer
You can see on the product datasheet, A15544 anti-MEGF9/Egfl5 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-03-22
Question
Can you help my question with product A15544, anti-MEGF9/Egfl5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Jones
Verified customer
Asked: 2013-04-16
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A15544 anti-MEGF9/Egfl5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-04-16
Question
I see that the anti-MEGF9/Egfl5 antibody A15544 works with WB, what is the protocol used to produce the result images on the product page?
N. Zhang
Verified customer
Asked: 2013-04-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-04-15
