Anti-MED27/Crsp8 Antibody

Product Name

Anti-MED27/Crsp8 Antibody

View all MED27 Antibodies

SKU/Catalog Number

A10484

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-MED27/Crsp8 Antibody (Catalog # A10484). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MED27/Crsp8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10484)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from Internal of human MED27.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10484 is reactive to MED27 in Human, Mouse

Reconstitution

Calculated molecular weight

35432 MW

Background of MED27

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Humphray S.J., Nature 429:369-374(2004).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Ryu S., Nature 397:446-450(1999).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10484 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For MED27 (Source: Uniprot.org, NCBI)

Alternative Names

Mediator of RNA polymerase II transcription subunit 27;Cofactor required for Sp1 transcriptional activation subunit 8;CRSP complex subunit 8;Mediator complex subunit 27;P37 TRAP/SMCC/PC2 subunit;Transcriptional coactivator CRSP34;MED27;CRSP34 , CRSP8; MED27 CRAP34, CRSP34, CRSP8, MED3, TRAP37 mediator complex subunit 27 mediator of RNA polymerase II transcription subunit 27|CRSP complex subunit 8|cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa|epididymis secretory sperm binding protein|p37 TRAP/SMCC/PC2 subunit|transcriptional coactivator CRSP34

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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