Anti-T-bet/Tbx21 Antibody

T-bet/TBX21 antibody

Boster Bio Anti-T-bet/Tbx21 Antibody catalog # PA1999. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PA1999
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-T-bet/Tbx21 Antibody

View all T-bet/TBX21 Antibodies

SKU/Catalog Number

PA1999

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-T-bet/Tbx21 Antibody catalog # PA1999. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-T-bet/Tbx21 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1999)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human T-bet, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1999 is reactive to TBX21 in Human, Mouse, Rat

Applications

PA1999 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

58-60 kDa

Calculated molecular weight

58.328kDa

Background of T-bet/TBX21

TBX21 (T-Box 21), also called TBET, is a protein that in humans is encoded by the TBX21 gene. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. Zhang and Yang (2000) mapped the mouse Tbx21 gene to chromosome 11D in an area showing homology of synteny with human chromosome 17. Szabo et al. (2000) showed that TBX21 expression correlates with IFNG expression in Th1 and natural killer (NK) cells. Ectopic expression of TBX21 both transactivated the IFNG gene and induced endogenous IFNG production. Finotto et al. (2002) observed reduced expression of the T (H)1 transcription factor Tbet in T cells from airways of patients with asthma compared with that in T cells from airways of nonasthmatic patients, suggesting that loss of Tbet might be associated with asthma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For TBX21 (Source: Uniprot.org, NCBI)

Gene Name

TBX21

Full Name

T-box transcription factor TBX21

Weight

58.328kDa

Alternative Names

Tbet; T-bet; TBETT-PET; TBLYM; TBLYMT-cell-specific T-box transcription factor T-bet; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; TBX21; T-PET; Transcription factor TBLYM TBX21 T-PET, T-bet, TBET, TBLYM T-box transcription factor 21 T-box transcription factor TBX21|T-box 21|T-box expressed in T cells|T-box protein 21|T-cell-specific T-box transcription factor T-bet|transcription factor TBLYM

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TBX21, check out the TBX21 Infographic

TBX21 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TBX21: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1999 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Miao L, Run-Ming J, Yi J. Iran J Pediatr. 2012 Mar;22(1):43-51. T-Bet Mediated Anti-Neoplastic Effects Of Dendritic Cell-Cytokine Induced Killer Cells In Vitro.

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6 Customer Q&As for Anti-T-bet/Tbx21 Antibody

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-T-bet/Tbx21 antibody PA1999. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-05-08

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-05-08

Question

I see that the anti-T-bet/Tbx21 antibody PA1999 works with WB, what is the protocol used to produce the result images on the product page?

D. Walker

Verified customer

Asked: 2020-03-19

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-19

Question

Would anti-T-bet/Tbx21 antibody PA1999 work for WB with leukocyte?

A. Wu

Verified customer

Asked: 2018-11-06

Answer

According to the expression profile of leukocyte, TBX21 is highly expressed in leukocyte. So, it is likely that anti-T-bet/Tbx21 antibody PA1999 will work for WB with leukocyte.

Boster Scientific Support

Answered: 2018-11-06

Question

Would PA1999 anti-T-bet/Tbx21 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-10-06

Answer

You can see on the product datasheet, PA1999 anti-T-bet/Tbx21 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-10-06

Question

We are currently using anti-T-bet/Tbx21 antibody PA1999 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?

N. Banerjee

Verified customer

Asked: 2014-01-16

Answer

The anti-T-bet/Tbx21 antibody (PA1999) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-01-16

Question

Our lab want to know about to test anti-T-bet/Tbx21 antibody PA1999 on human leukocyte for research purposes, then I may be interested in using anti-T-bet/Tbx21 antibody PA1999 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

C. Lewis

Verified customer

Asked: 2014-01-15

Answer

The products we sell, including anti-T-bet/Tbx21 antibody PA1999, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-01-15

Order DetailsPrice
PA1999

100μg

$370
PA1999-10ug

10μg sample (liquid)

$99
PA1999-Biotin

100 μg Biotin conjugated

$570
PA1999-Cy3

100 μg Cy3 conjugated

$570
PA1999-Dylight488

100 μg Dylight488 conjugated

$570
PA1999-Dylight550

100 μg Dylight550 conjugated

$570
PA1999-Dylight594

100 μg Dylight594 conjugated

$570
PA1999-FITC

100 μg FITC conjugated

$570
PA1999-HRP

100 μg HRP conjugated

$570
PA1999-APC

100 μg APC conjugated

$670
PA1999-PE

100 μg PE conjugated

$670
PA1999-carrier-free

Carrier Free

$370

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