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Product Info Summary
| SKU: | M01180-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CRM1 DyLight® 488 conjugated XPO1 Antibody (monoclonal, 5G3)
SKU/Catalog Number
M01180-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human CRM1 DyLight® 488 conjugated XPO1 Antibody (monoclonal, 5G3) catalog # M01180-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human CRM1 DyLight® 488 conjugated XPO1 Antibody (monoclonal, 5G3) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01180-Dyl488)
Host
Mouse
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Monoclonal
Clone Number
5G3
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human CRM1 recombinant protein (Position: N966-D1071). Human CRM1 shares 93.4% and 91.5% amino acid (aa) sequence identity with mouse and rat CRM1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01180-Dyl488 is reactive to XPO1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
123.386kDa
Background of CRM1
Exportin 1 (XPO1), also known as chromosomal maintenance 1 (CRM1), is an eukaryotic protein that mapped to human chromosome 2p16 by fluorescence in situ hybridization. This protein mediates leucine-rich nuclear export signal (NES)-dependent protein transport. It specifically inhibits the nuclear export of Rev and U snRNAs. Additionally, this protein is involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. It also regulates NFAT and AP-1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01180-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
FCM: K562 cell
Validation Images & Assay Conditions
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Figure 1. Flow Cytometry analysis of K562 cells using anti-Human CRM1 antibody (M01180-Dyl488).
Overlay histogram showing K562 cells stained with M01180-Dyl488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Human CRM1 Antibody (M01180-Dyl488,1μg/1x106 cells) for 30 min at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For XPO1 (Source: Uniprot.org, NCBI)
Gene Name
XPO1
Full Name
Exportin-1
Weight
123.386kDa
Superfamily
exportin family
Alternative Names
Exportin-1; Exp1; Chromosome region maintenance 1 protein homolog; XPO1; CRM1 XPO1 CRM-1, CRM1, emb, exp1 exportin 1 exportin-1|chromosome region maintenance 1 homolog|chromosome region maintenance 1 protein homolog|exportin 1 (CRM1 homolog, yeast)|exportin-1 (required for chromosome region maintenance)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on XPO1, check out the XPO1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for XPO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CRM1 DyLight® 488 conjugated XPO1 Antibody (monoclonal, 5G3) (M01180-Dyl488)
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