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Product Info Summary
| SKU: | A00054 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-MDM2 (F414) Antibody
SKU/Catalog Number
A00054
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MDM2 (F414) Antibody catalog # A00054. Tested in WB,IHC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MDM2 (F414) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00054)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide, corresponding to amino acids 385-430 of Human MDM2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00054 is reactive to MDM2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
55233 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00054 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:50-1:200
IF: 1:50-1:200
Validation Images & Assay Conditions
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Figure 2. Immunohistochemistry validation of MDM2 using Anti-MDM2 (F414) Antibody (A00054).
Immunohistochemistry (IHC) analyzes of MDM2 (F414) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.For more protocol information of IHC
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Figure 1. Western blotting validation for Anti-MDM2 (F414) Antibody A00054
Western blot (WB) analysis of MDM2 (F414) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate(40ug)
Lane2:MCF-7 whole cell lysate(40ug)
Lane3:H9C2 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Click image to see more details
Figure 3. Western blotting validation for Anti-MDM2 (F414) Antibody A00054
Western blot (WB) analysis of MDM2 (F414) pAb at 1:500 dilutionLane1:HCC827 whole cell lysate(40ug)
Lane2:U-87MG whole cell lysate(40ug)
Lane3:HCT116 whole cell lysate(40ug)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For MDM2 (Source: Uniprot.org, NCBI)
Gene Name
MDM2
Full Name
E3 ubiquitin-protein ligase Mdm2
Weight
55233 MW
Superfamily
MDM2/MDM4 family
Alternative Names
E3 ubiquitin-protein ligase Mdm2;2.3.2.27 MDM2 ACTFS, HDMX, LSKB, hdm2 MDM2 proto-oncogene E3 ubiquitin-protein ligase Mdm2|MDM2 oncogene, E3 ubiquitin protein ligase|MDM2 proto-oncogene, E3 ubiquitin protein ligase|Mdm2, p53 E3 ubiquitin protein ligase homolog|Mdm2, transformed 3T3 cell double minute 2, p53 binding protein|double minute 2, human homolog of; p53-binding protein|oncoprotein Mdm2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MDM2, check out the MDM2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MDM2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MDM2 (F414) Antibody (A00054)
Hello CJ!
A00054 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jiang X,Yuan J,Dou Y,Zeng D, Xiao S.Lipopolysaccharide Affects the Proliferation and Glucose Metabolism of Cervical Cancer Cells Through the FRA1/MDM2/p53 Pathway.Int J Med Sci 2021;18(4): 1030-1038.doi:10. 7150/ijms.47360.
Species: Human
A00054 usage in article: APP:WB, SAMPLE:CASKI CELL AND HELA CELL, DILUTION:NA
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