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Product Info Summary
| SKU: | A08842 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Mdm2-binding protein MTBP Antibody
SKU/Catalog Number
A08842
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Mdm2-binding protein MTBP Antibody (Catalog # A08842). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
MTBP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Mdm2-binding protein MTBP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08842)
Host
Rabbit
Contents
MTBP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
MTBP antibody was raised against a 18 amino acid peptide near the center of human MTBP. The immunogen is located within amino acids 110 - 160 of MTBP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
C2CD3 antibody is human, mouse and rat reactive.
Reactive Species
A08842 is reactive to MTBP in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
102193 MW
Background of MTBP
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2 and MTBP, were recently discovered. The transcriptional activity of p53 is modulated by posttranslational regulations of the p53 protein including stabilization and acetylation. P53 transcriptionally activates MDM2 gene then the translated MDM2 protein binds to p53 and promotes the degradation of p53 leading to lowering the concentration of p53 protein. MDM2 inhibits both p53 mediated G1 arrest and apoptosis. A recently discovered protein termed MTBP was found to bind to MDM2 and to inhibit the modulation effect of MDM2 on p53. MTBP is expressed in a variety of normal tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08842 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MTBP antibody can be used for detection of MTBP by Western blot at 1 to 2 μg/mL. A 104 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MTBP expression in K562 cell lysate with MTBP antibody at (A) 1 and (B) 2 μg/ml.
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Immunocytochemistry of MTBP in K562 cells with MTBP antibody at 5 μg/mL.
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Immunofluorescence of MTBP in K562 cells with MTBP antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For MTBP (Source: Uniprot.org, NCBI)
Gene Name
MTBP
Full Name
Mdm2-binding protein
Weight
102193 MW
Superfamily
MTBP family
Alternative Names
MDM2BP, Mdm2-binding protein, hMTBP MTBP MDM2BP MDM2 binding protein mdm2-binding protein|MDM2 (mouse double minute 2)-binding protein, 104kD|Mdm2, transformed 3T3 cell double minute 2, p53 binding protein binding protein, 104kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MTBP, check out the MTBP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MTBP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mdm2-binding protein MTBP Antibody (A08842)
Hello CJ!
A08842 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jiang X,Yuan J,Dou Y,Zeng D, Xiao S.Lipopolysaccharide Affects the Proliferation and Glucose Metabolism of Cervical Cancer Cells Through the FRA1/MDM2/p53 Pathway.Int J Med Sci 2021;18(4): 1030-1038.doi:10. 7150/ijms.47360.
Species: Human
A08842 usage in article: APP:WB, SAMPLE:CASKI CELL AND HELA CELL, DILUTION:NA
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