Click image to see more details
Product Info Summary
| SKU: | A02571 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MBD3 Antibody
SKU/Catalog Number
A02571
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-MBD3 Antibody (Catalog # A02571). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MBD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02571)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human MBD3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02571 is reactive to MBD3 in Human, Mouse
Reconstitution
Calculated molecular weight
32844 MW
Background of Mbd3
Acts as transcriptional repressor and plays a role in gene silencing. Does not bind to DNA by itself (. Binds to DNA with a preference for sites containing methylated CpG dinucleotides (in vitro). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (). Recruits histone deacetylases and DNA methyltransferases.
Hendrich B., Mol. Cell. Biol. 18:6538-6547(1998).
Grimwood J., Nature 428:529-535(2004).
Tatematsu K., Genes Cells 5:677-688(2000).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02571 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells, using MBD3 antibody A02571.
Protein Target Info & Infographic
Gene/Protein Information For MBD3 (Source: Uniprot.org, NCBI)
Gene Name
MBD3
Full Name
Methyl-CpG-binding domain protein 3
Weight
32844 MW
Alternative Names
Methyl-CpG-binding domain protein 3;Methyl-CpG-binding protein MBD3;MBD3; Mbd3|AI181826, AU019209|methyl-CpG binding domain protein 3|methyl-CpG-binding domain protein 3|methyl-CpG-binding protein MBD3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MBD3, check out the MBD3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MBD3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MBD3 Antibody (A02571)
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No publications found for A02571
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Customer Q&As
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4 Customer Q&As for Anti-MBD3 Antibody
Question
I see that the anti-MBD3 antibody A02571 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-MBD3 antibody A02571. Let me know if you need anything else.
E. Patel
Verified customer
Asked: 2019-01-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-08
Question
I was wanting to use your anti-MBD3 antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
G. Wu
Verified customer
Asked: 2013-09-18
Answer
It shows on the product datasheet, A02571 anti-MBD3 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-09-18
Question
Please see the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-MBD3 antibody A02571. Please let me know if you require anything else.
N. Walker
Verified customer
Asked: 2013-07-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-07-23
