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Product Info Summary
| SKU: | M01746 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MBD2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01746
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MBD2 Rabbit Monoclonal Antibody catalog # M01746. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MBD2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01746)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADIH-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MBD2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01746 is reactive to MBD2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
56 kDa
Calculated molecular weight
32520 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01746 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: A375 cell
Validation Images & Assay Conditions
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Western blot analysis of MBD2 expression in A375 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MBD2 (Source: Uniprot.org, NCBI)
Gene Name
MBD2
Full Name
Methyl-CpG-binding domain protein 2
Weight
32520 MW
Alternative Names
G1/S-specific cyclin-D3;CCND3; MBD2 DMTase, NY-CO-41 methyl-CpG binding domain protein 2 methyl-CpG-binding domain protein 2|demethylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MBD2, check out the MBD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MBD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MBD2 Rabbit Monoclonal Antibody (M01746)
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No publications found for M01746
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Customer Q&As
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6 Customer Q&As for Anti-MBD2 Rabbit Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M01746?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of MBD2 expression in A375 cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
We need to test anti-MBD2 Monoclonal antibody M01746 on mouse testis for research purposes, then I may be interested in using anti-MBD2 Monoclonal antibody M01746 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
The products we sell, including anti-MBD2 Monoclonal antibody M01746, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-30
Question
Would anti-MBD2 Monoclonal antibody M01746 work for WB with testis?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
According to the expression profile of testis, MBD2 is highly expressed in testis. So, it is likely that anti-MBD2 Monoclonal antibody M01746 will work for WB with testis.
Boster Scientific Support
Answered: 2019-10-15
Question
Is this M01746 anti-MBD2 Monoclonal antibody reactive to the isotypes of MBD2?
J. Johnson
Verified customer
Asked: 2017-12-27
Answer
The immunogen of M01746 anti-MBD2 Monoclonal antibody is A synthesized peptide derived from human MBD2 Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemimethylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-27
Question
Would M01746 anti-MBD2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
O. Li
Verified customer
Asked: 2017-01-24
Answer
As indicated on the product datasheet, M01746 anti-MBD2 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-01-24
Question
Is a blocking peptide available for product anti-MBD2 Monoclonal antibody (M01746)?
T. Banerjee
Verified customer
Asked: 2015-06-12
Answer
We do provide the blocking peptide for product anti-MBD2 Monoclonal antibody (M01746). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-06-12
