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Product Info Summary
| SKU: | A00169-4 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Mavs Antibody Picoband®
SKU/Catalog Number
A00169-4
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Mavs Antibody Picoband® catalog # A00169-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Mavs Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00169-4)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Mavs recombinant protein (Position: M1-Q77).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00169-4 is reactive to Mavs in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
57 kDa
Calculated molecular weight
56528 MW
Background of MAVS
Mitochondrial antiviral-signaling protein (MAVS) is a protein that in humans is encoded by the MAVS gene. The protein is also known by the names VISA (virus-induced signaling adapter), IPS-1 and Cardif. This gene encodes an intermediary protein necessary in the virus-triggered beta interferon signaling pathways. It is required for activation of transcription factors which regulate expression of beta interferon and contributes to antiviral immunity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00169-4 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
ELISA, 0.1-0.5 μg/ml, Mouse
Positive Control
WB: rat brain tissue, rat heart tissue, rat L6 whole cell, mouse brain tissue, mouse HEPA1-6 whole cell, mouse C2C12 whole cell
FCM: ANA-1 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Mavs using anti-Mavs antibody (A00169-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: rat L6 whole cell lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse HEPA1-6 whole cell lysates,
Lane 6: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mavs antigen affinity purified polyclonal antibody (Catalog # A00169-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Mavs at approximately 57 kDa. The expected band size for Mavs is at 57 kDa.
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Figure 2. Flow Cytometry analysis of ANA-1 cells using anti-Mavs antibody (A00169-4).
Overlay histogram showing ANA-1 cells stained with A00169-4 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Mavs Antibody (A00169-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Mavs (Source: Uniprot.org, NCBI)
Gene Name
Mavs
Full Name
Mitochondrial antiviral-signaling protein
Weight
56528 MW
Alternative Names
Mitochondrial antiviral-signaling protein;MAVS;CARD adapter inducing interferon beta;Cardif;Interferon beta promoter stimulator protein 1;IPS-1;Putative NF-kappa-B-activating protein 031N;Virus-induced-signaling adapter;VISA;MAVS;IPS1, KIAA1271, VISA; MAVS CARDIF, IPS-1, IPS1, VISA mitochondrial antiviral signaling protein mitochondrial antiviral-signaling protein|CARD adapter inducing interferon beta|CARD adaptor inducing IFN-beta|IFN-B promoter stimulator 1|interferon beta promoter stimulator protein 1|putative NF-kappa-B-activating protein 031N|virus-induced signaling adaptor|virus-induced-signaling adapter
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Mavs, check out the Mavs Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Mavs: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mavs Antibody Picoband® (A00169-4)
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