Anti-Matrix metalloproteinase-9 MMP9 Antibody

Product Name

Anti-Matrix metalloproteinase-9 MMP9 Antibody

View all MMP-9 Antibodies

SKU/Catalog Number

A00139

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Matrix metalloproteinase-9 MMP9 Antibody (Catalog # A00139). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

MMP9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Cite This Product

Anti-Matrix metalloproteinase-9 MMP9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00139)

Host

Rabbit

Contents

MMP9 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

MMP9 antibody was raised against a 16 amino acid peptide near the center of human MMP9. The immunogen is located within amino acids 260 - 310 of MMP9 .

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

MMP9 antibody is human, mouse and rat reactive. At least three isoforms of MMP9 are known to exist; this antibody only recognizes the two longest isoforms.

Reactive Species

A00139 is reactive to MMP9 in Human, Mouse, Rat

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

78458 MW

Background of MMP-9

The matrix metalloproteinase (MMP) family are a family of proteins that are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases (1). MMP9 degrades type IV and V collagens (2) and studies suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow (3) and plays a role in tumor-associated tissue remodeling (4).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

A00139 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

MMP9 antibody can be used for detection of MMP9 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For MMP9 (Source: Uniprot.org, NCBI)

Alternative Names

GELB, CLG4B, MMP-9, MANDP2, Matrix metalloproteinase-9, 92 kDa gelatinase MMP9 CLG4B, GELB, MANDP2, MMP-9 matrix metallopeptidase 9 matrix metalloproteinase-9|macrophage gelatinase|matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|type V collagenase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis