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Product Info Summary
| SKU: | A04203-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MAT1A Antibody Picoband®
SKU/Catalog Number
A04203-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MAT1A Antibody Picoband® catalog # A04203-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-MAT1A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04203-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MAT1A, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04203-1 is reactive to MAT1A in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
87794 MW
Background of MAT1A
This gene catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. The encoded protein is found as a homotetramer (MAT I) or a homodimer (MAT III) whereas a third form, MAT II (gamma), is encoded by the MAT2A gene. Mutations in this gene are associated with methionine adenosyltransferase deficiency.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04203-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Positive Control
WB: rat liver tissue, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MAT1A using anti-MAT1A antibody (A04203-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAT1A antigen affinity purified polyclonal antibody (Catalog # A04203-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAT1A at approximately 55 kDa. The expected band size for MAT1A is at 44 kDa.
Protein Target Info & Infographic
Gene/Protein Information For MAT1A (Source: Uniprot.org, NCBI)
Gene Name
MAT1A
Full Name
S-adenosylmethionine synthase isoform type-1
Weight
87794 MW
Superfamily
AdoMet synthase family
Alternative Names
Transforming acidic coiled-coil-containing protein 1;Gastric cancer antigen Ga55;Taxin-1;TACC1;KIAA1103; MAT1A MAT, MATA1, SAMS, SAMS1 methionine adenosyltransferase 1A S-adenosylmethionine synthase isoform type-1|MAT 1|MAT-I/III|adoMet synthase 1|adoMet synthetase 1|methionine adenosyltransferase 1|methionine adenosyltransferase I, alpha|methionine adenosyltransferase I/III
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAT1A, check out the MAT1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAT1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAT1A Antibody Picoband® (A04203-1)
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