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Product Info Summary
| SKU: | PA1648 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Monoamine Oxidase A/MAOA Antibody Picoband®
SKU/Catalog Number
PA1648
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Monoamine Oxidase A/MAOA Antibody catalog # PA1648. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Monoamine Oxidase A/MAOA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1648)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MAOA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1648 is reactive to MAOA in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
59682 MW
Background of MAO-A
MAOA (Monoamine oxidase A), also known as AMINE OXIDASE (FLAVIN-CONTAINING) A, is an enzyme that in humans is encoded by the MAO-A gene. MAOA is an isozyme of monoamine oxidase which is also mapped on Xp11.3. MAOA degrades amine neurotransmitters, such as dopamine, norepinephrine, and serotonin. The protein localizes to the outer mitochondrial membrane. Mutation in MAOA results in monoamine oxidase deficiency, or Brunner syndrome. In humans, there is a 30-base repeat sequence repeated in one of several different numbers of times in the promoter region of the gene coding for MAOA. MAO-A levels in the brain as measured using positron emission tomography are elevated by an average of 34% in patients with major depressive disorder. Inhibition of MAOA prevented apoptosis, and serum starvation of cortical brain cells from Maoa-deficient mice resulted in reduced apoptosis compared with wildtype mice.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1648 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human RT4 whole cell, human placenta tissue, rat heart tissue, rat liver tissue, rat kidney tissue, mouse kidney tissue
IHC: Human Placenta Tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MAOA using anti-MAOA antibody (PA1648).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human RT4 whole cell lysates,
Lane 2: human placenta tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat liver tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAOA antigen affinity purified polyclonal antibody (Catalog # PA1648) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAOA at approximately 65 kDa. The expected band size for MAOA is at 60 kDa.
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Anti-MAOA antibody, PA1648, IHC(P)
IHC(P): Human Placenta Tissue
Protein Target Info & Infographic
Gene/Protein Information For MAOA (Source: Uniprot.org, NCBI)
Gene Name
MAOA
Full Name
Amine oxidase [flavin-containing] A
Weight
59682 MW
Superfamily
flavin monoamine oxidase family
Alternative Names
Amine oxidase [flavin-containing] A;1.4.3.4;Monoamine oxidase type A;MAO-A;MAOA; MAOA BRNRS, MAO-A monoamine oxidase A amine oxidase [flavin-containing] A|monoamine oxidase type A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAOA, check out the MAOA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAOA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Monoamine Oxidase A/MAOA Antibody Picoband® (PA1648)
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No publications found for PA1648
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Customer Q&As
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4 Customer Q&As for Anti-Monoamine Oxidase A/MAOA Antibody Picoband®
Question
I see that the anti-Monoamine Oxidase A/MAOA antibody PA1648 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-25
Question
Does anti-Monoamine Oxidase A/MAOA antibody PA1648 work for IHC with liver?
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
According to the expression profile of liver, MAOA is highly expressed in liver. So, it is likely that anti-Monoamine Oxidase A/MAOA antibody PA1648 will work for IHC with liver.
Boster Scientific Support
Answered: 2018-01-05
Question
Is a blocking peptide available for product anti-Monoamine Oxidase A/MAOA antibody (PA1648)?
A. Mangal
Verified customer
Asked: 2015-11-24
Answer
We do provide the blocking peptide for product anti-Monoamine Oxidase A/MAOA antibody (PA1648). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-11-24
Question
Does anti-Monoamine Oxidase A/MAOA antibody PA1648 work on dog IHC with placenta?
F. Lewis
Verified customer
Asked: 2013-03-28
Answer
Our lab technicians have not tested anti-Monoamine Oxidase A/MAOA antibody PA1648 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Monoamine Oxidase A/MAOA antibody PA1648 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog placenta in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-03-28
