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Product Info Summary
| SKU: | EK1676 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human TRKC ELISA Kit PicoKine®
SKU/Catalog Number
EK1676
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TRKC ELISA Kit PicoKine™ (96 Tests). Quantitate Human NTRK3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml .
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TRKC ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1676)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: C32-A428
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1676 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1676 is reactive to NTRK3 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1676 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TrkC
Tropomyosin receptor kinase C (TrkC), also known as NT-3 growth factor receptor, neurotrophic tyrosine kinase receptor type 3, or TrkC tyrosine kinase is a protein that in humans is encoded by the NTRK3 gene.This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. Several transcript variants encoding different isoforms have been found for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1676-CAP | Anti-Human NTRK3 Pre-coated 96-well strip microplate | 1 |
| EK1676-ST | Human NTRK3 Standard | 2 vials, 10 ng/tube |
| EK1676-DA | Human NTRK3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.036 | 0.062 | 0.124 | 0.197 | 0.319 | 0.579 | 1.049 | 1.947 |
Data Example Images
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Human TRKC PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TrkC, Dilution ratio of 1:1, concentration in serum and plasma is 1-1.6 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TRKC ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 330 | 1741 | 4941 | 335 | 1753 | 4967 |
| Standard deviation | 18.15 | 85.3 | 390.33 | 22.78 | 92.9 | 466.89 |
| CV (%) | 5.5% | 4.9% | 7.9% | 6.8% | 5.3% | 9.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TrkC in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 330 | 342 | 297 | 342 | 327 | 18.41 | 5.6% |
| Sample 2 | 1741 | 1582 | 1616 | 1731 | 1667 | 69.63 | 4.1% |
| Sample 3 | 4941 | 4243 | 4806 | 4622 | 4653 | 262.39 | 5.6% |
Protein Target Info & Infographic
Gene/Protein Information For NTRK3 (Source: Uniprot.Org, NCBI)
Gene Name
NTRK3
Full Name
NT-3 growth factor receptor
Weight
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.10; EC 2.7.10.1; ETS related protein-neurotrophic receptor tyrosine kinase fusion protein; ETV6-NTRK3 fusion; gp145(trkC); GP145-TrkC; Neurotrophic tyrosine kinase receptor type 3; neurotrophic tyrosine kinase, receptor, type 3; NT 3 receptor; NT-3 growth factor receptor; NTRK3; TrkC tyrosine kinase; TrkC; trk-C; TRKCneurotrophin 3 receptor; tyrosine kinase receptor C NTRK3 GP145-TrkC, TRKC, gp145(trkC) neurotrophic receptor tyrosine kinase 3 NT-3 growth factor receptor|ETS related protein-neurotrophic receptor tyrosine kinase fusion protein|ETV6-NTRK3 fusion|neurotrophic tyrosine kinase, receptor, type 3|tyrosine kinase receptor C
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NTRK3, check out the NTRK3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NTRK3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TRKC ELISA Kit PicoKine® (EK1676)
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Customer Q&As
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8 Customer Q&As for Human TRKC ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated TrkC antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TrkC antigen?
Verified Customer
Verified customer
Asked: 2020-12-16
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-12-16
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-11-13
Question
Q: What is the optimal O.D. value for TrkC ELISA kit? I used your TrkC ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TrkC even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TrkC ELISA kit to have sensitivity of 10pg/ml , that means the minimum amount of TrkC that can be declared/interpreted as positive by the above standard is 10pg/ml .
Boster Scientific Support
Answered: 2019-08-19
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of TrkC in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-06-08
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TrkC ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-06-08
Question
Q: can I use citrate plasma as samples in Human TrkC Picokine® ELISA Kit (Catalog # EK1676)?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of TrkC causing degradation of its protein structure. TrkC may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TrkC ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-05-15
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-01-23
Question
Q: how to proceed with the analysis of ELISA data? I have performed TrkC level in plasma.
W. Campbell
Verified customer
Asked: 2015-09-25
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2015-09-25
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
T. Carter
Verified customer
Asked: 2014-01-19
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2014-01-19
