Click image to see more details
Product Info Summary
| SKU: | M03570 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Dog, Human, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal Antibody
SKU/Catalog Number
M03570
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal Antibody (Catalog # M03570). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Rat, Dog.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03570)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM282
Isotype
IgG1, kappa
Immunogen
Human MAGE-A1 full length recombinant protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M03570 is reactive to MAGEA1 in Dog, Human, Rat
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
34342 MW
Background of MAGE 1
Recognizes a protein of 42-46kDa, identified as MAGE-1. This monoclonal antibody does not cross-react with MAGE-2, -3, -4, -6 -9, -10, -or -12 protein. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03570 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Testis stained with Anti-MAGE-1 Monoclonal Antibody (SPM282).
Protein Target Info & Infographic
Gene/Protein Information For MAGEA1 (Source: Uniprot.org, NCBI)
Gene Name
MAGEA1
Full Name
Melanoma-associated antigen 1
Weight
34342 MW
Alternative Names
Melanoma-associated antigen 1;Antigen MZ2-E;Cancer/testis antigen 1.1;CT1.1;MAGE-1 antigen;MAGEA1;MAGE1, MAGE1A; MAGEA1 CT1.1, MAGE1 MAGE family member A1 melanoma-associated 1|MAGE-1 | MZ2-E|cancer/testis 1.1|cancer/testis family 1, member 1|melanoma MAGE-1|melanoma family A 1|melanoma family A, 1 (directs expression of MZ2-E)|melanoma family A1|melanoma-associated MZ2-E
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAGEA1, check out the MAGEA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAGEA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal Antibody (M03570)
Hello CJ!
No publications found for M03570
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal Antibody
Question
We are currently using anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody M03570 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says canine, human, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
The anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody (M03570) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-10
Question
I have a question about product M03570, anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03570 anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-19
Question
See below the WB image, lot number and protocol we used for testis using anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody M03570. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-07
Question
Is a blocking peptide available for product anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody (M03570)?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
We do provide the blocking peptide for product anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody (M03570). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-04-11
Question
I see that the anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody M03570 works with IHC, what is the protocol used to produce the result images on the product page?
J. Bhatt
Verified customer
Asked: 2018-07-09
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-07-09
Question
Will M03570 anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Taylor
Verified customer
Asked: 2015-10-07
Answer
It shows on the product datasheet, M03570 anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-10-07
Question
Will anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody M03570 work for IHC with testis?
N. Mitchell
Verified customer
Asked: 2014-08-13
Answer
According to the expression profile of testis, MAGEA1 is highly expressed in testis. So, it is likely that anti-MAGE-1 (Target for Cancer Immunotherapy) Monoclonal antibody M03570 will work for IHC with testis.
Boster Scientific Support
Answered: 2014-08-13
