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Product Info Summary
| SKU: | A08829 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Maelstrom Antibody
SKU/Catalog Number
A08829
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Maelstrom Antibody (Catalog # A08829). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maelstrom antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Maelstrom Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08829)
Host
Rabbit
Contents
Maelstrom Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Maelstrom antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human Maelstrom. The immunogen is located within amino acids 30 - 80 of Maelstrom.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
CADM3 antibody is human, mouse and rat reactive. At least three isoforms are known to exist; this antibody will only detect the two longest isoforms. CADM3 antibody is predicted to not cross-react with other members of the CADM protein family.
Reactive Species
A08829 is reactive to MAEL in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
49219 MW
Background of MAEL
The mammalian homolog of the Drosophila protein Maelstrom is expressed in the male germline and localizes to the sex body in spermatocytes and the chromatoid body in round spermatids. Similar to its expression in Drosophila, Maelstrom is a component of nuages, a germ-cell specific organelle and is thought to be essential for spermatogenesis and transposon repression during meiosis. In humans, Maelstrom has been found to be expressed only in the testis and in various cancer cell lines. Treatment of these cell lines with the demethylating agent 5'-Aza-2-Deoxycytidine significantly upregulated Maelstrom levels, indicating that its expression is regulated by DNA methylation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08829 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Maelstrom antibody can be used for detection of Maelstrom by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Maelstrom in HeLa cell lysate with Maelstrom antibody at 1 μg/mL.
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Immunocytochemistry of Maelstrom in HeLa cells with Maelstrom antibody at 5 μg/mL.
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Immunofluorescence of Maelstrom in HeLa cells with Maelstrom antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For MAEL (Source: Uniprot.org, NCBI)
Gene Name
MAEL
Full Name
Protein maelstrom homolog
Weight
49219 MW
Superfamily
maelstrom family
Alternative Names
CT128, SPATA35, Protein maelstrom homolog MAEL CT128, SPATA35 maelstrom spermatogenic transposon silencer protein maelstrom homolog|cancer/testis 128|maelstrom homolog|spermatogenesis associated 35|testicular tissue protein Li 116
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAEL, check out the MAEL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAEL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Maelstrom Antibody (A08829)
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