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Product Info Summary
| SKU: | A02357 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-MAD2L2/Mad2B Antibody
SKU/Catalog Number
A02357
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-MAD2L2/Mad2B Antibody (Catalog # A02357). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-MAD2L2/Mad2B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02357)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-terminus region of Human MAD2L2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02357 is reactive to MAD2L2 in Human
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
24334 MW
Background of MAD2L2
MAD2L2 is also known as MAD2B, REV7 and mitotic arrest deficient-like 2 protein. MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02357 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:7,500 - 1:30,000
IHC: 1:500 - 1:2,000
WB: 1:500 - 1:2,000
Validation Images & Assay Conditions
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MAD2L2 was detected in paraffin-embedded sections of human kidney tissues using rabbit anti-MAD2L2 Antigen Affinity purified polyclonal antibody (Catalog # A02357). Brown color indicates presence of protein, blue color shows cell nuclei. Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
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Western blot analysis of MAD2L2 expression in HeLa whole cell lysates (lane 1) and nuclear lysates (lane 2). MAD2L2 at 20KD was detected using rabbit anti-MAD2L2 Antigen Affinity purified polyclonal antibody (Catalog # A02357) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For MAD2L2 (Source: Uniprot.org, NCBI)
Gene Name
MAD2L2
Full Name
Mitotic spindle assembly checkpoint protein MAD2B
Weight
24334 MW
Alternative Names
MAD2L2, MAD2B, REV7, hREV7, REV homolog, MAD2-like protein 2, Mitotic arrest deficient 2-like protein 2, Mitotic spindle assembly checkpoint protein MAD2B MAD2L2 FANCV, MAD2B, POLZ2, REV7 mitotic arrest deficient 2 like 2 mitotic spindle assembly checkpoint protein MAD2B|MAD2 (mitotic arrest deficient, yeast, homolog)-like 2|MAD2 mitotic arrest deficient-like 2|MAD2-like protein 2|REV7 homolog|hREV7|mitotic arrest deficient 2-like protein 2|mitotic arrest deficient homolog-like 2|polymerase (DNA-directed), zeta 2, accessory subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAD2L2, check out the MAD2L2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAD2L2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MAD2L2/Mad2B Antibody (A02357)
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