Click image to see more details
Product Info Summary
| SKU: | A04513-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Lunatic Fringe LFNG Antibody
View all Lunatic Fringe Antibodies
SKU/Catalog Number
A04513-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Lunatic Fringe LFNG Antibody catalog # A04513-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Lunatic Fringe LFNG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04513-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human LFNG. AA range:121-170
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04513-1 is reactive to LFNG in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
41773 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04513-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of 3T3 cells using Lunatic Fringe Polyclonal Antibody diluted at 1:1000
Protein Target Info & Infographic
Gene/Protein Information For LFNG (Source: Uniprot.org, NCBI)
Gene Name
LFNG
Full Name
Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe
Weight
41773 MW
Superfamily
glycosyltransferase 31 family
Alternative Names
Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe;2.4.1.222;O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase;LFNG; LFNG SCDO3 LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase beta-1,3-N-acetylglucosaminyltransferase lunatic fringe
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LFNG, check out the LFNG Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LFNG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Lunatic Fringe LFNG Antibody (A04513-1)
Hello CJ!
No publications found for A04513-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Lunatic Fringe LFNG Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Lunatic Fringe LFNG Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Anti-Lunatic Fringe LFNG Antibody
Question
Is this A04513-1 anti-Lunatic Fringe antibody reactive to the isotypes of LFNG?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
The immunogen of A04513-1 anti-Lunatic Fringe antibody is Synthesized peptide derived from the Internal region of human Lunatic Fringe. at AA rangle: 90-170. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-13
Question
See below the WB image, lot number and protocol we used for vagina using anti-Lunatic Fringe antibody A04513-1. Please let me know if you require anything else.
K. Mitchell
Verified customer
Asked: 2019-12-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-24
Question
My question regards to test anti-Lunatic Fringe antibody A04513-1 on rat vagina for research purposes, then I may be interested in using anti-Lunatic Fringe antibody A04513-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The products we sell, including anti-Lunatic Fringe antibody A04513-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for vagina using anti-Lunatic Fringe antibody A04513-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-25
Question
Does A04513-1 anti-Lunatic Fringe antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Patel
Verified customer
Asked: 2018-11-29
Answer
It shows on the product datasheet, A04513-1 anti-Lunatic Fringe antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-29
Question
I see that the anti-Lunatic Fringe antibody A04513-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-06
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-11-06
Question
Will anti-Lunatic Fringe antibody A04513-1 work on goat WB with vagina?
Verified Customer
Verified customer
Asked: 2018-04-30
Answer
Our lab technicians have not tested anti-Lunatic Fringe antibody A04513-1 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Lunatic Fringe antibody A04513-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat vagina in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-30
Question
I was wanting to use your anti-Lunatic Fringe antibody for WB for rat vagina on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat vagina identification?
Verified Customer
Verified customer
Asked: 2017-12-14
Answer
It shows on the product datasheet, A04513-1 anti-Lunatic Fringe antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat vagina in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-14
Question
Is a blocking peptide available for product anti-Lunatic Fringe antibody (A04513-1)?
V. Anderson
Verified customer
Asked: 2017-09-18
Answer
We do provide the blocking peptide for product anti-Lunatic Fringe antibody (A04513-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-09-18
Question
Do you have a BSA free version of anti-Lunatic Fringe antibody A04513-1 available?
Verified Customer
Verified customer
Asked: 2017-09-15
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Lunatic Fringe antibody A04513-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-09-15
Question
Can you help my question with product A04513-1, anti-Lunatic Fringe antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-05
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04513-1 anti-Lunatic Fringe antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-05
Question
We are currently using anti-Lunatic Fringe antibody A04513-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
C. Taylor
Verified customer
Asked: 2014-09-04
Answer
The anti-Lunatic Fringe antibody (A04513-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-09-04
Question
Will anti-Lunatic Fringe antibody A04513-1 work for WB with vagina?
C. Bhatt
Verified customer
Asked: 2013-09-02
Answer
According to the expression profile of vagina, LFNG is highly expressed in vagina. So, it is likely that anti-Lunatic Fringe antibody A04513-1 will work for WB with vagina.
Boster Scientific Support
Answered: 2013-09-02
